Z2126

ZAP-70 Tyrosine Kinase human

≥90% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells (Histidine tagged), buffered aqueous glycerol solution, ≥150 U/mg

• MDL number: MFCD03459122
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected insect cells (Histidine tagged)
• Quality Level: 200
• assay: ≥90% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ≥150 U/mg
• mol wt: 70 kDa
• UniProt accession no.: P43403
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... ZAP70(7535)

Description

Application

Human ZAP-70 Tyrosine Kinase was used for mapping the phosphorylation sites on LAT (linker for activation of T cells) for investigating the mechanism of activation of signalling proteins in T cells.

Biochem/physiol Actions

ZAP-70 Tyrosine Kinase is a protein belongs to Syk family and is essential for T cell activation. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. Additionally, it also plays a pivotal role in LFA-1-dependent T Cell Migration.

ZAP-70 is protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. It is required for T cell activation. It is activated by Lck-mediated phosphorylation of its tyrosine residues.

Unit Definition

One unit will phosphorylate 1 nmol polyglutamic acid:tyrosine per min at 37 °C at pH 7.0.

Physical form

Solution in 25 mM HEPES, pH 7.5, containing 150 mM NaCl, 0.5 mM DTT, and 50% glycerol.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1