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ZMS1050

Sigma-Aldrich

Anti-Brn-3a Antibody, clone 5A3.2 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5A3.2, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 42.7 kDa
observed mol wt ~50 kDa

purified by

using Protein A

species reactivity

human

species reactivity (predicted by homology)

rat, chicken

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

epitope sequence

Internal

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 5A3.2 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects Brn-3a. It targets an epitope within 39 amino acids from the internal region.

Immunogen

Recombinant fusion protein fragment corresponding to 39 amino acids from the internal region of human Brn-3a.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human in Brn-3a.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human Brn-3a.

Tested applications

ELISA Analysis: 300 μg/mL from a representative lot detected recombinant human Brn-3a.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Brn-3a in human retina tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Brn-3a, clone 5A3.2 ZooMAb®, Cat. No. ZMS1050, is a recombinant Mouse monoclonal antibody that detects Brn-3a and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

POU domain, class 4, transcription factor 1 (UniProt: Q01851; also known as Brain-specific homeobox/POU domain protein 3A, Brain-3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1) is encoded by the POU4F1 (also known as BRN3A, RDC1) gene (Gene ID: 5457) in human. Brn-3a is a multifunctional transcription factor that is expressed in the brain and the retina and is present in the developing brain, spinal cord, and eye. Its C-terminal domain is able to act as both DNA-binding domain and a transcriptional activator. Its N-terminal domain is shown to be essential for its transactivation activity on some target genes. Brn-3a also activates Bcl-2 expression and protects neuronal cells from apoptosis. It regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been reported that neurite outgrowth and expression of genes required for synapse formation are primarily dependent on the C-terminal domain, however the N-terminal domain is required for maximal induction. Two isoforms of Brn-3a have been described that are produced by alternative splicing. Isoform 1 is shown to interact with Brn-3b (POU4F2) and this interaction inhibits both DNA-binding and transcriptional activities of Brn3a. Isoform 2 that lacks amino acids 1-84 can act as transcription factor but cannot regulate the expression of the same subset of genes and does not have antiapoptotic effects on neuronal cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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