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ZMS1083

Sigma-Aldrich

Anti-HBV X-Protein Antibody, clone 227 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

227, recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 16.58 kDa
observed mol wt ~18 kDa

purified by

using protein G

species reactivity

hepatitis B virus

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

ELISA: suitable
affinity binding assay: suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

C-terminal half

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

vaccinia virus ... X(944566)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 227 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects X Protein of Hepatitis B virus. It targets an epitope within 26 amino acids from the C-terminal half.

Immunogen

A linear peptide corresponding to 26 amino acids from the C-terminal half of X Protein of Hepatitis B virus (HBV).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Protein X from HBV-271.]

Western Blotting Analysis (WB): A 1:20,000 dilution of this antibody detected recombinant X-Protein of HBV-271.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound HBV X-Protein with a KD of 7.0 x 10-9 in an affinity binding assay.

Enzyme Immunoassay Analysis (ELISA): A representative lot of this antibody detected recombinant X-protein of Hepatitis B virus (HBV) in ELISA applications

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Protein X (UniProt: P69713; also known as HBx, Peptide X, pX) is encoded by the X gene (Gene ID: 944566) in Hepatitis B virus. Hepatitis B virus (HBV) contains seven main proteins, which include Core, pre-Core, Small S, Middle S, Large S, Polymerase, and the HBx protein. HBx, a multifunctional protein, although not essential for HBV infection, plays a role in silencing host antiviral defenses and promoting viral transcription. It facilitates the efficient replication of HBV by stimulating HBV gene expression from the cDNA template. It is mainly localized in the cytoplasm with a small fraction detected in the nucleus. However, its expression level can also influence its cellular localization. It is predominantly nuclear when expressed in cells at very low levels but becomes largely cytoplasmic as its expression level increases. In cytoplasm, a minor fraction may also associate with mitochondria. Its mitochondrial targeting sequence is localized in amino acids 68-117. HBx has an N-terminal negative regulatory domain and its transactivation or coactivation of the C-terminal domain is reported to interfere with host cell signal transduction transduction pathways to help HBV replication. The best-characterized HBx binding partner is the cellular damage-specific DNA binding protein 1 (DDB1) and the interaction of HBx-DDB1 is essential for HBV replication. HBx promotes the degradation of the cellular structural maintenance of chromosomes 5/6 complex (Smc5/6), which directly binds DNA and entraps DNA plasmids. HBx is directly involved in development of cirrhosis and liver and hepatocellular carcinoma. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kornyeyev, D., et al. (2019). J. Virol. 93(16); e00248-19; Hwang, G-Y., et al. (2003). J. Clin. Microbiol. 41(12); 5598-5603).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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