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ZMS5406

Sigma-Aldrich

Anti-GAD67 Antibody, clone 1G10.2 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform

biological source

mouse (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G10.2, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 66.9 kDa
observed mol wt ~58 kDa

species reactivity

mouse, rat

species reactivity (predicted by homology)

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG2a

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GAD1(2571)

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ZRB1091ZRB1310ZRB1008
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Gene Information

human ... GAD1(2571)

Gene Information

human ... GAD2(2572)

Gene Information

human ... GRIA1(2890)
mouse ... Gria1(14799)
rat ... Gria1(50592)

Gene Information

rat ... Gria2;Glur2(29627)

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

1G10.2, recombinant monoclonal

clone

3B23, recombinant monoclonal

clone

1C11, monoclonal, recombinant monoclonal

clone

1K4, recombinant monoclonal

species reactivity

mouse, rat

species reactivity

rat, mouse, human

species reactivity

human, mouse, rat

species reactivity

mouse, human, rat

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1G10.2 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Glutamate decarboxylase 1 (GAD67).

Immunogen

Recombinant human Glutamate decarboxylase 1 (GAD67).

Application

Anti-GAD67, clone 1G10.2 ZooMAb, Cat. No. ZMS5406, is a recombinant Mouse monoclonal antibody that specifically targets Glutamate decarboxylase 1 (GAD67) and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAD67 in mouse brain tissue lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAD67 in rat E18 cortical cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GAD67 in mouse brain and rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Glutamate decarboxylase 1 (UniProt: Q99259; also known as EC:4.1.1.15, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform) is encoded by the GAD1 (also known as GAD, GAD67) gene (Gene ID:2571) in human. Glutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD have been described (65 kDa and 67 kDa) that display 64% amino acid homology and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct, and ovary. Between the two isoforms, GAD67 is responsible for over 90% of basal GABA synthesis and is produced at limiting levels in the brain. These isoforms are regionally distributed in cytoplasm in the brains of rats and mice. GAD67 expression is shown to change during neural development in rat spinal cord and its expression seems to be particularly plastic and can change in response to experimental manipulation and disease progression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
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Related Content

Choose sustainable options for your research with greener alternative ZooMAb® recombinant antibodies. These BSA-free, carrier-free antibodies are shipped in low-waste packaging and offer maximum stability without compromising performance.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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