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G5419

Sigma-Aldrich

Monoclonal Anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody produced in mouse

clone K-87, purified antibody

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Synonym(s):
Anti-GAD 67
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

K-87, monoclonal

mol wt

antigen 67 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:20,000 (with antigen-retrieval step)
western blot: 1:500-1:20,000 using mouse brain extract

isotype

IgG1κ

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

human ... GAD1(2571)
mouse ... Gad1(14415)
rat ... Gad1(24379)

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This Item
G1166SAB4200232G4913
Gene Information

human ... GAD1(2571)
mouse ... Gad1(14415)
rat ... Gad1(24379)

Gene Information

human ... GAD2(2572)
mouse ... Gad2(14417)

Gene Information

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

Gene Information

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

IgG fraction of antiserum

clone

K-87, monoclonal

clone

GAD-6, monoclonal

clone

N-GAD65, monoclonal

clone

polyclonal

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General description

Glutamic Acid Decarboxylase 67 (GAD67) is encoded by the gene glutamic acid decarboxylase 1 (GAD1). GAD1 gene is located on the human chromosome at 2q31.1. GAD67 is localized in the dendrites and cell bodies.

Specificity

Reacts specifically with GAD67. Does not crossreact with GAD65.

Immunogen

This peptide does not share homology with GAD65.
synthetic peptide from mouse GAD67 (amino acids 87-106).

Application

Monoclonal Anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody is suitable for use in immunoblot, western blot (at 1:500-1:20,000 dilutions using mouse brain extract) and immunohistochemistry (at 1:500-1:20,000 dilutions in mouse brain sections).
Monoclonal anti-glutamic acid decarboxylase 67 (GAD67) antibody produced in mouse has been used in:
  • immunohistofluorescence
  • western blotting
  • immunohistochemistry.

Biochem/physiol Actions

Glutamic Acid Decarboxylase 67 (GAD67) is involved in the production of γ-amino butyric acid (GABA), a inhibitory neurotransmitter in the central nervous system (CNS). Deficiency of GAD67 leads to psychosis, schizophrenia and bipolar diseases.

Physical form

Solution in phosphate buffered saline containing 0.01% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Mitsuhiro Tsuboi et al.
Cancer medicine, 8(9), 4189-4199 (2019-06-18)
In a previous genome-wide screening, we identified hypermethylated CpG islands around glutamate decarboxylase 1 (GAD1) in lung adenocarcinoma (LADC). In this study, we aimed to investigate the methylation and expression status of GAD1 and its prognostic value in patients with
Minjung Kim et al.
PloS one, 7(2), e32043-e32043 (2012-03-01)
The analysis of co-localized protein expression in a tissue section is often conducted with immunofluorescence histochemical staining which is typically visualized in localized regions. On the other hand, chromogenic immunohistochemical staining, in general, is not suitable for the detection of
Baohu Ji et al.
PloS one, 8(6), e66327-e66327 (2013-07-05)
It has been well established that schizophrenia patients display impaired NMDA receptor (NMDAR) functions as well as exacerbation of symptoms in response to NMDAR antagonists. Abnormal NMDAR signaling presumably contributes to cognitive deficits which substantially contribute to functional disability in
Silvia Rossi et al.
Brain, behavior, and immunity, 25(5), 947-956 (2010-10-14)
Synaptic dysfunction triggers neuronal damage in experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis (MS). While excessive glutamate signaling has been reported in the striatum of EAE, it is still uncertain whether GABA synapses are altered. Electrophysiological recordings showed
Ying Chen et al.
Neuropharmacology, 60(7-8), 1075-1087 (2010-09-28)
GAD67 corresponds to one of two enzymes that decarboxylates glutamate to produce γ-aminobutyric acid, the main inhibitory neurotransmitter in the mammalian central nervous system, hence defining the cellular phenotype of a diverse set of inhibitory interneurons of the brain. Reduced

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