ZRB04953
Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
A5S, recombinant monoclonal
monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 52.31 kDa
observed mol wt ~55 kDa
species reactivity
human
species reactivity (predicted by homology)
mouse, rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
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enhanced validation
recombinant expression
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sustainability
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technique(s)
immunocytochemistry: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:200
inhibition assay: suitable using 1:1,000 (peptide)
western blot: suitable using 1:1,000
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer465/pSer467)
Gene Information
human ... SMAD2;MADH2;MADR2(4087)
General description
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
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Specificity
Immunogen
Application
Immunohistochemistry (Paraffin) Analysis: A 1:200 and 1:100 dilution from a representative lot detected phospho-Smad2 (Ser465/467) in human breast cancer and human colon cancer tissue sections.
Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot was used with HeLa cells treated with TGF-beta for peptide block analysis.
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
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