ZRB1494
Anti-p-Ubiquitin (pSer65) Antibody, clone 2N21 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Ub
About This Item
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biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2N21, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
species reactivity
human
species reactivity (predicted by homology)
mouse, rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
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Design for Energy Efficiency
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer65)
General description
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Immunogen
Application
Flow Cytometry Analysis: 0.1 μg from a representative lot detected p-Ubiquitin (pSer65) in one million PC-3 cells treated with CCCP.
Peptide Inhibition Assay: Target band detection in lysate from HeLa cells transfected with PINK and treated with CCCP (15 mM, 5 h) was prevented by preblocking of a representative lot with the phosphorylated Ser 65 ubiquitin peptide, but not by corresponding non-phosphopeptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p-Ubiquitin (pSer65) in HeLa cells transfected with PINK and treated wtih CCCP.
Affinity Binding Assay: A representative lot of this antibody bound phosphorylated serine 65 Ubiquitin peptide with a KD of 2.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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