MilliporeSigma
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66828-U

Supelco

BIOshell A400 Protein C4, 3.4 μm HPLC Column

3.4 μm particle size, L × I.D. 10 cm × 4.6 mm

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NACRES:
SB.52

material

stainless steel hardware

Quality Level

Agency

suitable for USP L26

description

Shell thickness (0.2 μm)
Solid Core (3.0 μm)

product line

BIOshell

feature

endcapped

manufacturer/tradename

BIOshell

packaging

1 ea of

extent of labeling

0.4% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 4.6 mm

surface area

15 m2/g

surface coverage

4.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C4 (butyl) phase

particle size

3.4 μm

pore size

400 Å

operating pH range

2-9

separation technique

reversed phase

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This Item
66825-U66826-U66829-U
agency

suitable for USP L26

agency

suitable for USP L26

agency

suitable for USP L26

agency

suitable for USP L26

product line

BIOshell

product line

BIOshell

product line

BIOshell

product line

BIOshell

feature

endcapped

feature

endcapped

feature

endcapped

feature

endcapped

packaging

1 ea of

packaging

1 ea of

packaging

1 ea of

packaging

1 ea of

extent of labeling

0.4% carbon loading

extent of labeling

0.4% carbon loading

extent of labeling

0.4% carbon loading

extent of labeling

0.4% carbon loading

General description

BIOshell 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

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Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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Articles

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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