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Supelco

BIOshell A400 Protein C4 (3.4 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

Synonym(s):

Protein C4 Reversed-Phase Chromatography Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

BIOshell A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel hardware

Quality Level

agency

suitable for USP L26

description

Shell thickness (0.2 μm)
Solid Core (3.0 μm)

product line

BIOshell

feature

endcapped

manufacturer/tradename

BIOshell

packaging

1 ea of

extent of labeling

0.4% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

15 m2/g

surface coverage

4.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C4 (butyl) phase

particle size

3.4 μm

pore size

400 Å

operating pH range

2-9

separation technique

reversed phase

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General description

BIOshell 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.

The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.

A RPLC-MS workflow for mass analysis of reduced monoclonal antibody, trastuzumab, including reduction procedures, calibration, and system suitability tests.

A RPLC-MS workflow for mass analysis of non-reduced monoclonal antibody, trastuzumab, including calibration, and system suitability tests.

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Protocols

Larger porous shell particles with narrow particle size distribution, used in Supelco's BIOshell™ columns for the reversed-phase U/HPLC analysis of peptides and proteins, provide increased efficiency per unit pressure drop.

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4

Related Content

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

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