66825-U
BIOshell A400 Protein C4 (3.4 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Synonym(s):
Protein C4 Reversed-Phase Chromatography Column
About This Item
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product name
BIOshell A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm
material
stainless steel hardware
Quality Level
agency
suitable for USP L26
description
Shell thickness (0.2 μm)
Solid Core (3.0 μm)
product line
BIOshell
feature
endcapped
manufacturer/tradename
BIOshell
packaging
1 ea of
extent of labeling
0.4% carbon loading
parameter
600 bar max. pressure
90 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
15 m2/g
surface coverage
4.2 μmol/m2
matrix
spherical silica particle platform
superficially porous particle
matrix active group
C4 (butyl) phase
particle size
3.4 μm
pore size
400 Å
operating pH range
2-9
separation technique
reversed phase
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Articles
Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
A RPLC-MS workflow for mass analysis of reduced monoclonal antibody, trastuzumab, including reduction procedures, calibration, and system suitability tests.
A RPLC-MS workflow for mass analysis of non-reduced monoclonal antibody, trastuzumab, including calibration, and system suitability tests.
Protocols
Larger porous shell particles with narrow particle size distribution, used in Supelco's BIOshell™ columns for the reversed-phase U/HPLC analysis of peptides and proteins, provide increased efficiency per unit pressure drop.
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Related Content
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
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