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BIOshell lgG 1000 Å C4 (2.7 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

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Synonym(s):
BIOshell lgG C4 Chromatography
UNSPSC Code:
41115709
NACRES:
SB.52

product name

BIOshell lgG 1000 Å C4, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel hardware

Quality Level

agency

suitable for USP L26

description

Shell thickness (0.5 μm)
Solid core (1.7 μm)

product line

BIOshell

feature

endcapped

specific analyte(s)

IgG

manufacturer/tradename

BIOshell

packaging

1 ea of

extent of labeling

0.6% carbon loading

parameter

1000 bar pressure (14500 psi)
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

22 m2/g

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C4 (butyl) phase

particle size

2.7 μm

pore size

1000 Å

operating pH range

2-9

separation technique

reversed phase

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General description

BIOshell IgG 1000Å C4 is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000Å) Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5-micron thin porous shell and the small overall particle size of 2.7-microns. The densely bonded, extensively endcapped dimethylbutyl stationary phase of BIOshell IgG 1000Å C4 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds such as proteins.

Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Articles

BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Protocols

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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