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66905-U

Supelco

BIOshell A160 Peptide C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

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NACRES:
SB.52

material

stainless steel hardware

Quality Level

Agency

suitable for USP L1

description

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

product line

BIOshell
BIOshell

feature

endcapped: no

analyte chemical class(es)

peptides
peptides

manufacturer/tradename

BIOshell
BIOshell

packaging

1 ea of

extent of labeling

4.6% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

90 m2/g

surface coverage

2.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle
superficially porous particle

matrix active group

C18 (octadecyl) bonding phase, diisobutyloctadecyl

particle size

2.7 μm
2.7 μm

pore size

160 Å

operating pH range

1-8

separation technique

reversed phase
reversed phase

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This Item
66904-U66907-U66908-U
agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

product line

BIOshell

product line

BIOshell

product line

BIOshell

product line

BIOshell

feature

endcapped: no

feature

endcapped: no

feature

endcapped: no

feature

endcapped: no

packaging

1 ea of

packaging

1 ea of

packaging

1 ea of

packaging

1 ea of

extent of labeling

4.6% carbon loading

extent of labeling

4.6% carbon loading

extent of labeling

4.6% carbon loading

extent of labeling

4.6% carbon loading

General description

BIOshell 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected C18 ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C). This greatly expands the application range for the separation of biomolecules.

Application

BIOshell A160 Peptide C18, 2.7 μm HPLC Column, a non-end capped column that has superficially porous particle with C18 alkyl ligand as the stationary phase, is used mainly in the separation of peptides at a good flow rate.

Features and Benefits

BIOshell A160 Peptide C18, 2.7 μm HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than columns with fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

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Collagen-derived X-Hyp-Gly-type tripeptides promote differentiation of MC3T3-E1 pre-osteoblasts
Taga Y, et al.
Journal of functional foods, 46, 456-462 (2018)
Jennifer K Field et al.
Journal of chromatography. A, 1622, 461093-461093 (2020-04-29)
The Peptide RPC Column Characterisation Protocol was applied to 38 stationary phases, varying in ligand chemistry, base silica, end capping and pore size, which are suitable for the analysis of peptides. The protocol at low and intermediate pH is based

Articles

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

This application note describes a step-by-step LC-UV-MS workflow for teriparatide peptide mapping, that includes enzymatic digestion, separation conditions, and their high resolution QTOF mass spectrometer based identification.

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.

Protocols

Insulin is an approximately 6000 Da peptide hormone used to regulate the glucose concentration in blood. There are several different forms of insulin that are both natural and recombinant; the recombinant forms have been engineered to improve pharmacokinetic properties. A quick and reliable chromatographic technique was developed to identify and detect a mixture of insulin variants in an unknown sample.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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