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66969-U

Supelco

BIOshell A160 Peptide CN (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

BIOshell A160 Peptide CN, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel hardware (column)

Quality Level

agency

suitable for USP L10

description

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

product line

BIOshell

feature

endcapped

analyte chemical class(es)

peptides

manufacturer/tradename

BIOshell

packaging

1 ea of

extent of labeling

2.2% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

90 m2/g

surface coverage

2.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle

matrix active group

cyano phase

particle size

2.7 μm

pore size

160 Å

operating pH range

1-8

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

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General description

BIOshell 160Å Peptide CN columns provide an alternative selectivity to the traditional C18 bonded phases. The introduction of the cyano group enables peptides and small proteins to be separated due to a combination of hydrophobicity and dipole-dipole interactions making this column an excellent choice for biomolecules that are not resolved on traditional C18 columns. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected cyanopropyl ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C).

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Articles

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

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