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NIST8671

NISTmAb, Humanized IgG1k Monoclonal Antibody

NIST® SRM® 8671

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About This Item

UNSPSC Code:
41116107
NACRES:
NA.41

antibody product type

primary antibodies

grade

certified reference material

form

liquid

manufacturer/tradename

NIST®

technique(s)

UV/Vis spectroscopy: suitable
liquid chromatography (LC): suitable
mass spectrometry (MS): suitable

format

matrix material

General description

NISTmAb, Humanized IgG1k Monoclonal Antibody is a recombinant antibody expressed in murine suspension culture. It is a homodimer with two identical light chains and two identical heavy chains linked through inter- and intra- chain disulfide bonds with a molecular weight of 150 kDa. The protein possesses a low abundance of post-translational modifications including methionine oxidation, deamidation, and glycation. This humanized monoclonal antibody also has N-terminal pyroglutamination, C-terminal lysine clipping, and glycosylation of the heavy chains.

A unit of RM 8671 consists of one internal-threaded polypropylene cryovial containing 800 µL of 10 mg/mL NIST IgG1κ monoclonal antibody (NISTmAb) in 12.5 mmol/L L-histidine, 12.5 mmol/L L-histidine HCl (pH 6.0).

SRM 8671_Cert

SRM 8671_SDS

Application

NISTmAb, Humanized IgG1k Monoclonal Antibody has been used as a standard for testing de novo sequencing algorithms on bottom-up mass spectrometry.This reference material (RM) is primarily intended for evaluating the performance of methods for determining physiochemical and biophysical attributes of monoclonal antibodies. It also provides a representative test molecule for developing novel technology in therapeutic protein characterization. Other potential uses include:
  • system suitability tests
  • establishing method or instrument performance and variability
  • comparing changing analytical test methods
  • assisting in method qualification

Analysis Note

NIST8671 is intended for research use. It is not intended for animal or human consumption, clinical testing or therapeutic use.

Other Notes

  • RM 8671 is intended for research use.
  • Details on expiration, storage, safety, usage, and source are provided in the NIST certificate. 
  • Information on biomaterials, disposal, and transport is available in the SDS.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology

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Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

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Ioannis Karageorgos et al.
Biologicals : journal of the International Association of Biological Standardization, 50, 27-34 (2017-10-03)
Monoclonal antibody pharmaceuticals are the fastest-growing class of therapeutics, with a wide range of clinical applications. To assure their safety, these protein drugs must demonstrate highly consistent purity and stability. Key to these objectives is higher order structure measurements validated
Automated Antibody De Novo Sequencing and Its Utility in Biopharmaceutical Discovery.
Sen K I, et al.
Journal of the American Society For Mass Spectrometry, 28(5), 803-810 (2017)

Articles

An Optimized Protocol for Peptide Mapping of Therapeutic Monoclonal Antibodies with Minimum Deamidation and Oxidation Artifacts

An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.

Middle-Up Antibody Analysis by RP Chromatography

Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.

Charge Variant Analysis (CVA) with Strong Cation Exchange

Learn about the separation of several charge variants of two monoclonal antibodies, NISTmAb and Infliximab, using a polymeric cation exchange column and MS-compatible mobile phases.

A Comparison of Superficially Porous Particle-based Column Chemistries for Peptide Mapping

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

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Intact Mass Analysis of Monoclonal Antibodies

Discover our wide variety of products for intact mass analysis of monoclonal antibodies, including size-exclusion columns (SEC), ion exchange columns, reverse-phase columns, HPLC buffers, MALDI matrices and standards, high-purity solvents, reagents, tools for protein sample preparation, and certified reference materials.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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