G005614
HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18
application for HPLC
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About This Item
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technique(s)
HPLC: suitable
test parameters
column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: acetonitrile
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 290 nm
sample: 50 μg/mL each in acetonitrile
suitability
application for HPLC
application(s)
clinical
vitamins, nutraceuticals, and natural products
Analysis Note
This application demonstrates the suitability of the Ascentis C18 for the analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Ergocalciferol (D2), analytical standard
Cholecalciferol (D3), analytical standard
Standard
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Description
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analytical column
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