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  • Engineered Plant-Based Nanocellulose Hydrogel for Small Intestinal Organoid Growth.

Engineered Plant-Based Nanocellulose Hydrogel for Small Intestinal Organoid Growth.

Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2021-01-14)
Rodrigo Curvello, Genevieve Kerr, Diana J Micati, Wing Hei Chan, Vikram S Raghuwanshi, Joseph Rosenbluh, Helen E Abud, Gil Garnier
ABSTRACT

Organoids are three-dimensional self-renewing and organizing clusters of cells that recapitulate the behavior and functionality of developed organs. Referred to as "organs in a dish," organoids are invaluable biological models for disease modeling or drug screening. Currently, organoid culture commonly relies on an expensive and undefined tumor-derived reconstituted basal membrane which hinders its application in high-throughput screening, regenerative medicine, and diagnostics. Here, we introduce a novel engineered plant-based nanocellulose hydrogel is introduced as a well-defined and low-cost matrix that supports organoid growth. Gels containing 0.1% nanocellulose fibers (99.9% water) are ionically crosslinked and present mechanical properties similar to the standard animal-based matrix. The regulation of the osmotic pressure is performed by a salt-free strategy, offering conditions for cell survival and proliferation. Cellulose nanofibers are functionalized with fibronectin-derived adhesive sites to provide the required microenvironment for small intestinal organoid growth and budding. Comparative transcriptomic profiling reveals a good correlation with transcriptome-wide gene expression pattern between organoids cultured in both materials, while differences are observed in stem cells-specific marker genes. These hydrogels are tunable and can be combined with laminin-1 and supplemented with insulin-like growth factor (IGF-1) to optimize the culture conditions. Nanocellulose hydrogel emerges as a promising matrix for the growth of organoids.

MATERIALS
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Product Description

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