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Mast Cell Migration and Chemotaxis Assayed by Microscopy.

Methods in molecular biology (Clifton, N.J.) (2020-08-09)
Monika Bambousková, Zuzana Rubíková, Lubica Dráberová, Pavel Dráber, Petr Dráber
ABSTRACT

A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter, three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Albumin, dinitrophenyl, lyophilized powder
Sigma-Aldrich
Anti -Dinitrophenyl antibody, Mouse monoclonal, IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Fibronectin bovine plasma, solution, sterile-filtered, BioReagent, suitable for cell culture