MilliporeSigma
  • Comparative study on preparative methods of DC-Chol/DOPE liposomes and formulation optimization by determining encapsulation efficiency.

Comparative study on preparative methods of DC-Chol/DOPE liposomes and formulation optimization by determining encapsulation efficiency.

International journal of pharmaceutics (2012-05-31)
Shuoye Yang, Jiayin Chen, Di Zhao, Deen Han, Xijing Chen
ABSTRACT

Three most commonly used preparative methods, dry-film, reverse phase evaporation and ethanol injection were employed to prepare cationic liposomes composed of DC-Chol and DOPE, respectively. The resulting samples were contrasted through morphology observation, particle size and zeta potential analysis. Sephadex filtration method with high selectivity was developed to determine the encapsulation efficiency of plasmid DNA-loaded cationic vectors, on this basis, cationic liposomes formulation was further optimized by applying Box Behnken design with encapsulation efficiency as evaluation index. The results showed that liposomes prepared by dry-film method were of best quality and stability, moreover, the optimum formulation of cationic liposomes and optimal value of each influencing factors were quantitatively obtained, measured value was highly consistent with predicted results. These findings preliminarily clarified the effect of preparative methods on performance of cationic liposome, as well as formulation factors on encapsulation efficiency, and will provide important methodological reference for further study of liposomes carriers for gene delivery.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sephadex® G-15, Medium
Sigma-Aldrich
Sephadex® G-100, Superfine
Sigma-Aldrich
Sephadex® G-25, BioReagent, for molecular biology, DNA grade, Superfine
Sigma-Aldrich
Sephadex® G-100, BioReagent, for molecular biology, DNA grade, medium
Sigma-Aldrich
Sephadex® G-25, Medium
Sigma-Aldrich
Sephadex® G-75, Medium
Sigma-Aldrich
Sephadex® G-25, Coarse
Sigma-Aldrich
Sephadex® G-25, Fine
Sigma-Aldrich
Cholesteryl N-(2-dimethylaminoethyl)carbamate, ≥98% (TLC)
Sigma-Aldrich
Sephadex® G-75, Superfine
Sigma-Aldrich
Sephadex® G-100, Medium
Sigma-Aldrich
Sephadex® G-10, Medium
Sigma-Aldrich
Sephadex® G-50, Superfine
Sigma-Aldrich
Sephadex® G-50, Fine
Sigma-Aldrich
Sephadex® G-50, BioReagent, for molecular biology, DNA grade, medium
Supelco
Sephadex® G-50, Medium
Sigma-Aldrich
Sephadex® G-50, BioReagent, for molecular biology, DNA grade, fine