Inhibition of angiogenesis by antioxidant micelles.

Inhibition of angiogenesis by antioxidant micelles.

Advanced healthcare materials (2014-09-13)

Masaki Moriyama, Stéphanie Metzger, André J van der Vlies, Hiroshi Uyama, Martin Ehrbar, Urara Hasegawa

ABSTRACT

Antioxidant micelles capable of scavenging reactive oxygen species (ROS) are prepared from poly(ethylene glycol)-b-poly(dopamine) block copolymers. The micelles inhibit tube formation of human umbilical vein endothelial cells (HUVECs) by scavenging endogenous ROS. Furthermore, the micelles inhibit angiogenesis in the chicken ex ovo chorioallantoic membrane assay. The results show that antioxidant micelles containing catechol moieties may be useful in anti-angiogenic therapy to treat various diseases such as cancer.

MATERIALS

Product Number

Brand

Product Description

676853

Sigma-Aldrich

Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

D2050000

Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard

L092004

Sigma-Aldrich

Ethyl acetate

L092003

Sigma-Aldrich

Ethyl acetate

PHR1557

Supelco

Methylene Chloride, Pharmaceutical Secondary Standard; Certified Reference Material

PHR1481

Supelco

Ethyl Acetate, Pharmaceutical Secondary Standard; Certified Reference Material

50803

Supelco

Hexylamine, analytical standard

439169

Sigma-Aldrich

Ethyl acetate, suitable for HPLC, ≥99.8%

34856

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.8%, contains amylene as stabilizer

34869

Sigma-Aldrich

Dimethyl sulfoxide, suitable for HPLC, ≥99.7%

650528

Sigma-Aldrich

Ethyl acetate, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%

34858

Sigma-Aldrich

Ethyl acetate, suitable for HPLC, ≥99.7%

V900090

Sigma-Aldrich

Dimethyl sulfoxide, Vetec^™, reagent grade, 99%

I5161

SAFC

Iodoacetamide

27227

Sigma-Aldrich

Ethyl acetate, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99.5% (GC)

32222

Sigma-Aldrich

Dichloromethane, puriss. p.a., ACS reagent, reag. ISO, ≥99.9% (GC)

33211

Sigma-Aldrich

Ethyl acetate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

320269

Sigma-Aldrich

Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

D5879

Sigma-Aldrich

Dimethyl sulfoxide, ReagentPlus^®, ≥99.5%

D65100

Sigma-Aldrich

Dichloromethane, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

472301

Sigma-Aldrich

Dimethyl sulfoxide, ACS reagent, ≥99.9%

319902

Sigma-Aldrich

Ethyl acetate, ACS reagent, ≥99.5%

34943

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water

24233

Sigma-Aldrich

Dichloromethane, puriss., meets analytical specification of Ph. Eur., NF, ≥99% (GC)

41640

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)

494453

Sigma-Aldrich

Dichloromethane, biotech. grade, 99.9%, contains 40-150 ppm amylene as stabilizer

494518

Sigma-Aldrich

Ethyl acetate, biotech. grade, ≥99.8%

09-0730

Sigma-Aldrich

Ethyl acetate, suitable for HPLC

09-0690

Sigma-Aldrich

Ethyl acetate, SAJ first grade, ≥99.0%

18-1900

Sigma-Aldrich

L-Lysine monohydrochloride, SAJ special grade, ≥99.0%