Skip to Content
MilliporeSigma
  • Porous three-dimensional carbon nanotube scaffolds for tissue engineering.

Porous three-dimensional carbon nanotube scaffolds for tissue engineering.

Journal of biomedical materials research. Part A (2015-03-20)
Gaurav Lalwani, Anu Gopalan, Michael D'Agati, Jeyantt Srinivas Sankaran, Stefan Judex, Yi-Xian Qin, Balaji Sitharaman
ABSTRACT

Assembly of carbon nanomaterials into three-dimensional (3D) architectures is necessary to harness their unique physiochemical properties for tissue engineering and regenerative medicine applications. Herein, we report the fabrication and comprehensive cytocompatibility assessment of 3D chemically crosslinked macrosized (5-8 mm height and 4-6 mm diameter) porous carbon nanotube (CNT) scaffolds. Scaffolds prepared via radical initiated thermal crosslinking of single- or multiwalled CNTs (SWCNTs and MWCNTs) possess high porosity (>80%), and nano-, micro-, and macroscale interconnected pores. MC3T3 preosteoblast cells on MWCNT and SWCNT scaffolds showed good cell viability comparable to poly(lactic-co-glycolic) acid (PLGA) scaffolds after 5 days. Confocal live cell and immunofluorescence imaging showed that MC3T3 cells were metabolically active and could attach, proliferate, and infiltrate MWCNT and SWCNT scaffolds. SEM imaging corroborated cell attachment and spreading and suggested that cell morphology is governed by scaffold surface roughness. MC3T3 cells were elongated on scaffolds with high surface roughness (MWCNTs) and rounded on scaffolds with low surface roughness (SWCNTs). The surface roughness of scaffolds may be exploited to control cellular morphology and, in turn, govern cell fate. These results indicate that crosslinked MWCNTs and SWCNTs scaffolds are cytocompatible, and open avenues toward development of multifunctional all-carbon scaffolds for tissue engineering applications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chloroform, suitable for HPLC
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, SAJ super special grade, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, SAJ first grade, ≥99.0%, contains 0.4-0.8% ethanol
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, JIS 300, ≥99.0%, for residue analysis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, JIS special grade, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Anti-Vinculin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Luperox® A98, Benzoyl peroxide, reagent grade, ≥98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Supelco
Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 μL
Estimated to ship on March 21, 2025
$411.00
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Carbon nanotube, multi-walled, >90% carbon basis, D × L 110-170 nm × 5-9 μm
SKU
Pack Size
Availability
Price
Quantity