Skip to Content
MilliporeSigma
  • Structural characterization of amphiphilic siderophores produced by a soda lake isolate, Halomonas sp. SL01, reveals cysteine-, phenylalanine- and proline-containing head groups.

Structural characterization of amphiphilic siderophores produced by a soda lake isolate, Halomonas sp. SL01, reveals cysteine-, phenylalanine- and proline-containing head groups.

Extremophiles : life under extreme conditions (2015-10-07)
Luis O'mar Serrano Figueroa, Benjamin Schwarz, Abigail M Richards
ABSTRACT

Soap Lake, located in Washington State, is a naturally occurring saline and alkaline lake. Several organisms inhabiting this lake have been identified as producers of siderophores that are unique in structure. Bacterial isolates, enriched from Soap Lake sediment and water samples, were screened for siderophore production using both the chrome azurol S (CAS) agar plate and liquid methods. Bacterial isolate Halomonas sp. SL01 was found to produce relatively high concentrations of siderophores in liquid medium (up to 40 µM). Siderophores from the isolate were separated from the culture supernatant using solid phase extraction and purified by high-performance liquid chromatography (HPLC). Siderophore structure was determined using LC/MS/MS (liquid chromatography/mass spectrometry/mass spectrometry) and fatty acid methyl ester (FAME) GC. Two distinct new families of amphiphilic siderophores were produced by isolate SL01. All siderophores ranged in size from 989 to 1096 atomic mass units and consisted of a conserved peptidic head group (per family), which coordinates iron, coupled to fatty acid moieties. The fatty acyl moieties were C10-C14 in length and some with hydroxyl substitutions at the third α position. These siderophores resembled amphiphilic aquachelin siderophores produced by Halomonas aquamarina strain DS40M3, a marine bacterium as well as siderophores from isolate Halomonas sp. SL28 that was found to produce amphiphilic siderophores. Bacteria thriving under saline and alkaline conditions are capable of producing unique siderophores resembling those produced by microbes inhabiting marine environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium chloride solution, BioUltra, for molecular biology, ~1 M in H2O
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Potassium chloride, ≥99.99% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Potassium chloride, 99.999% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Ammonium chloride, 99.998% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Potassium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium nitrate, BioUltra, ≥99.0% (T)
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium nitrate, 99.995% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride solution, 0.85%
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride, 99.999% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Ammonium chloride, 99.99% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Potassium chloride, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Potassium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium pyruvate, ReagentPlus®, ≥99%
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium pyruvate, powder, BioXtra, suitable for mouse embryo cell culture
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium pyruvate, Hybri-Max, powder, suitable for hybridoma
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Ammonium chloride, for molecular biology, suitable for cell culture, ≥99.5%
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride, tablet
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium nitrate, ≥99.0%, suitable for plant cell culture
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium nitrate, BioXtra, ≥99.0%
Pricing and Inventory is not available. Please check the Millipore website for more information.
Supelco
Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride solution, 5 M
Pricing and Inventory is not available. Please check the Millipore website for more information.
Sigma-Aldrich
Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Pricing and Inventory is not available. Please check the Millipore website for more information.