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  • Analysis of 5-hydroxymethylfurfural in foods by gas chromatography-mass spectrometry.

Analysis of 5-hydroxymethylfurfural in foods by gas chromatography-mass spectrometry.

Journal of chromatography. A (2006-10-03)
E Teixidó, F J Santos, L Puignou, M T Galceran
ABSTRACT

A new, simple and selective method for the analysis of 5-hydroxymethylfurfural (HMF) in foods by gas chromatography coupled to mass spectrometry (GC-MS) is proposed. Several derivatising procedures based on the formation of an HMF silylated derivative using different reagents were studied. Among the derivatising reagents examined, N,O-bis-trimethylsilyltrifluoroacetamide (BSTFA) provided the best derivatisation yield. Sample clean-up was also optimised, using either liquid-liquid extraction with dichloromethane or solid-phase extraction (SPE) with several commercially available cartridges, and the best results were obtained using ENV+ cartridges. Quality parameters such as day-to-day and run-to-run precision (RSD<10%), linearity (between 25 and 700 ng g(-1)) and detection limit (6 ng g(-1)) were established. This method was successfully applied to the analysis of HMF content in several Spanish food samples from a local market, such as jam, honey, orange juice and bakery products.

MATERIALS
Product Number
Brand
Product Description

Supelco
N-Methyl-N-(trimethylsilyl)trifluoroacetamide, BioReagent, for silylations, LiChropur
Supelco
MSTFA Reagent, ampule of 10 × 1.2 mL, analytical standard, Cerilliant®
Supelco
N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane, for GC derivatization, LiChropur
Supelco
N-Methyl-N-(trimethylsilyl)trifluoroacetamide, synthesis grade
Supelco
N-Methyl-N-(trimethylsilyl)trifluoroacetamide, for GC derivatization, LiChropur, ≥98.5%
Sigma-Aldrich
N,O-Bis(trimethylsilyl)trifluoroacetamide, ≥99%