MilliporeSigma
  • A human immunoglobulin lambda locus is similarly well expressed in mice and humans.

A human immunoglobulin lambda locus is similarly well expressed in mice and humans.

The Journal of experimental medicine (1999-05-20)
A V Popov, X Zou, J Xian, I C Nicholson, M Brüggemann
ABSTRACT

Transgenic mice carrying a 380-kb region of the human immunoglobulin (Ig) lambda light (L) chain locus in germline configuration were created. The introduced translocus on a yeast artificial chromosome (YAC) accommodates the most proximal Iglambda variable region (V) gene cluster, including 15 Vlambda genes that contribute to >60% of lambda L chains in humans, all Jlambda-Clambda segments, and the 3' enhancer. HuIglambdaYAC mice were bred with animals in which mouse Igkappa production was silenced by gene targeting. In the kappa-/- background, human Iglambda was expressed by approximately 84% of splenic B cells. A striking result was that human Iglambda was also produced at high levels in mice with normal kappa locus. Analysis of bone marrow cells showed that human Iglambda and mouse Igkappa were expressed at similar levels throughout B cell development, suggesting that the Iglambda translocus and the endogenous kappa locus rearrange independently and with equal efficiency at the same developmental stage. This is further supported by the finding that in hybridomas expressing human Iglambda the endogenous L chain loci were in germline configuration. The presence of somatic hypermutation in the human Vlambda genes indicated that the Iglambda-expressing cells function normally. The finding that human lambda genes can be utilized with similar efficiency in mice and humans implies that L chain expression is critically dependent on the configuration of the locus.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
IgM, Kappa from murine myeloma, clone TEPC 183, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
IgG1, Kappa from murine myeloma, clone MOPC 21, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
IgG2a, Lambda from murine myeloma, clone HOPC-1, purified immunoglobulin, buffered aqueous solution