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MilliporeSigma

HPLC Analysis of Pain Relievers on Ascentis® Express C18

HPLC Analysis of Pain Relievers on Ascentis® Express C18 application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 5 μm HPLC Column

5 μm particle size, L × I.D. 10 cm × 4.6 mm

Standard

Product No.
Description
Pricing

Caffeine

meets USP testing specifications, anhydrous

Aspirin

meets USP testing specifications

Acetaminophen

meets USP testing specifications, 98.0-102.0%, powder

CONDITIONS

column

Ascentis Express C18, 10 cm x 4.6 mm I.D., 5 μm particles (50536-U)

mobile phase

[A] water with 3% acetic acid; [B] methanol with 3% acetic acid; (79:21, A:B)

flow rate

2.0 mL/min

pressure

3290 psi (227 bar)

column temp.

45 °C

detector

UV,275 nm

injection

10 μL

sample

100 - 360 μg/mLin mobile phase

Description

Analysis Note

This application shows the improvements in efficiency that may be obtained for USP methods using modern Fused-Core technology.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany