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HPLC Analysis of Protein Standards (1) on BIOshell A400 Protein C4

HPLC Analysis of Protein Standards (1) on BIOshell A400 Protein C4 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

BIOshell A400 Protein C4, 3.4 μm HPLC Column

3.4 μm particle size, L × I.D. 10 cm × 2.1 mm

Guard cartridge

Product No.
Description
Pricing

BIOshell A400 Protein C4, 3.4 μm Guard Cartridge

3.4 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 ea

Standard

Product No.
Description
Pricing

β-Galactosidase from Escherichia coli

For use as a marker in SDS-PAGE

L-Lactic Dehydrogenase from rabbit muscle

Type XI, lyophilized powder, 600-1,200 units/mg protein

Albumin from chicken egg white

For use as a marker in SDS-PAGE

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Conalbumin from chicken egg white

Substantially iron-free

Haptoglobin Human, Phenotype 1-1

98-100%, essentially salt-free, lyophilized powder

Trypsin Inhibitor from Glycine max (soybean)

BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

CONDITIONS

column

BIOshell A400 Protein C4, 10 cm X 2.1 mm, 3.4 μm particles (66825-U)

mobile phase

(A) 75:25, [0.1% TFA in water]:[0.1% TFA in acetonitrile] ; (B) 25:75, [0.1% TFA in water]:[0.1% TFA in acetonitrile]

gradient

25 to 65% B in 8 min

flow rate

0.3 mL/min

pressure

1430 psi (99 bar)

detector

UV, 215 nm

injection

1 μL

sample

protein mixture, g/L ea: lysozyme, 0.1; haptoglobulin, 0.3; conalbumin, 0.3; trypsin inhibitor, 0.3; glutamate dehydrogenase, 0.8; beta-galactosidase, 0.7; ovalbumin, 0.8; lactate dehydrogenase, 0.4

Description

Analysis Note

This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.

Legal Information

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