HPLC Analysis of Protein Standards (1) on BIOshell A400 Protein C4
Materials
analytical column
Product No.
Description
Pricing
guard cartridge
Product No.
Description
Pricing
BIOshell A400 Protein C4, 3.4 μm Guard Cartridge
3.4 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 eastandard
Product No.
Description
Pricing
L-Lactic Dehydrogenase from rabbit muscle
Type XI, lyophilized powder, 600-1,200 units/mg proteinTrypsin Inhibitor from Glycine max (soybean)
BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)CONDITIONS
column
BIOshell A400 Protein C4, 10 cm X 2.1 mm, 3.4 μm particles (66825-U)
mobile phase
(A) 75:25, [0.1% TFA in water]:[0.1% TFA in acetonitrile] ; (B) 25:75, [0.1% TFA in water]:[0.1% TFA in acetonitrile]
gradient
25 to 65% B in 8 min
flow rate
0.3 mL/min
pressure
1430 psi (99 bar)
detector
UV, 215 nm
injection
1 μL
sample
protein mixture, g/L ea: lysozyme, 0.1; haptoglobulin, 0.3; conalbumin, 0.3; trypsin inhibitor, 0.3; glutamate dehydrogenase, 0.8; beta-galactosidase, 0.7; ovalbumin, 0.8; lactate dehydrogenase, 0.4
Description
Analysis Note
This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.
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