Patient-Derived Organoid Biobanks for Drug Discovery

HUB Organoids BV is a pioneering biotechnology company specializing in the application of Organoid Technology to drug discovery, personalized medicine, and disease modeling. Founded in 2013 following the discovery of adult stem cells, the drug development platform from HUB uses advanced 3D organoid models derived from patient tissues, enabling more accurate and predictive research for preclinical testing and therapeutic development. This innovative platform accelerates the discovery of effective treatments by closely mimicking human biology and is available to scientists working in drug discovery and development in the therapeutic areas of oncology, inflammation and inflammatory bowel disease (IBD), infectious diseases, and toxicology investigations.

Section Overview

• Gastrointestinal Patient-Derived Organoids
• Lung Patient-Derived Organoids
• Pancreatic Patient-Derived Organoids

Gastrointestinal Patient-Derived Organoids

Our extensive biobank of normal and diseased tissue-derived human gastrointestinal organoids includes over 50 highly characterized intestinal organoids derived from multiple regions of the digestive systems such as stomach, rectum, small intestine (duodenum, ileum), and colon. The biobank contains 58 patient samples of multiple ages and genders with high viability that were cultured in 3dGRO^® L-WRN Conditioned Media.

Normal Gastrointestinal PDOs

ID	Catalog Number	Age	Sex	Organ	Disease Type or Normal
Prep 78-C	SCC311	52	F	Colon	Normal (RNA-seq)
Prep 80-C	SCC312	29	F	Colon	Normal (RNA-seq)
Prep 81-C	SCC313	49	F	Colon	Normal (RNA-seq)
Prep 81-D	SCC314	49	F	Duodenum	Normal (RNA-seq)
Prep 83-C	SCC315	45	F	Colon	Normal (RNA-seq)
Prep 83-D	SCC316	45	F	Duodenum	Normal (RNA-seq)
Prep 84-C	SCC317	56	F	Colon	Normal (RNA-seq)
Prep 84-D	SCC318	56	F	Duodenum	Normal (RNA-seq)
Prep 85-C	SCC319	62	F	Colon	Normal (RNA-seq)
Prep 85-D	SCC320	62	F	Duodenum	Normal (RNA-seq)
Prep 87-C	SCC321	21	M	Colon	Normal (RNA-seq)
Prep 87-D	SCC322	21	M	Duodenum	Normal (RNA-seq)
Prep 88-C	SCC323	33	F	Colon	Normal (RNA-seq)
Prep 88-D	SCC324	33	F	Duodenum	Normal (RNA-seq)
Prep 89-C	SCC325	55	M	Colon	Normal (RNA-seq)
Prep 89-D	SCC326	55	M	Duodenum	Normal (RNA-seq)
18-1417-N	SCC327	45	M	Colon	Normal (CRC tissue adjacent) (RNA-seq)
ht-099-C	SCC330	59	F	Colon	Normal (RNA-seq)
ht-101-C	SCC332	59	F	Colon	Normal (RNA-seq)
ht-103-C	SCC335	63	M	Colon	Normal (RNA-seq)
ht-104-C	SCC336	51	M	Colon	Normal (RNA-seq)
ht-104-I	SCC337	51	M	Ileum	Normal (RNA-seq)
ht-105-C	SCC338	19	M	Colon	Normal (RNA-seq)
ht-105-I	SCC339	19	M	Ileum	Normal (RNA-seq)
ht-118-C	SCC340	43	M	Colon	Normal
ht-119-C	SCC341	53	F	Colon	Normal
ht-127-S	SCC342	52	F	Stomach	Normal
ht-128-S	SCC343	60	F	Stomach	Normal
ht-129-S	SCC344	60	M	Stomach	Normal
ht-130-S	SCC345	68	M	Stomach	Normal
ht-215-CD	SCC346	59	F	Colon Descending	Normal
ht-215-CT	SCC347	59	F	Colon Transcending	Normal
ht-215-D	SCC348	59	F	Duodenum	Normal
ht-222-D	SCC349	33	M	Duodenum	Normal
ht-180-D	SCC350	38	F	Duodenum	Normal
ht-185-D	SCC351	45	M	Duodenum	Normal
ht-188-D	SCC352	50	F	Duodenum	Normal
ht-205-CS	SCC353	33	F	Colon Sigmoid	Normal Margin to Crohns (RNA-seq)
ht-205-D	SCC354	33	F	Duodenum	Normal Margin to Crohns
ht-205-I	SCC355	33	F	Ileum	Normal Margin to Crohns
ht-213-CA	SCC356	36	F	Colon Ascending	Normal margin to UC
ht-213-CD	SCC357	36	F	Colon Descending	Normal margin to UC
ht-213-CT	SCC358	36	F	Colon Transcending	Normal margin to UC
ht-213-D	SCC359	36	F	Duodenum	Normal margin to UC
ht-214-I	SCC360	46	M	Ileum	Normal margin to UC
ht-227-D	SCC362	62	M	Duodenum	Normal margin to UC
ht-230-D	SCC363	59	F	Duodenum	Normal margin to UC
ht-506-I	SCC368	34	F	Ileum	Normal Margin to Crohns
ht-352-D	SCC370	55	F	Duodenum	Normal
CSC.406N	SCC725	69	F	Colon	Normal (CRC tissue adjacent)
CSC.421N	SCC726	83	F	Colon	Normal (CRC tissue adjacent)
CSC.449N	SCC727	40	M	Colon	Normal (CRC tissue adjacent)

Adenoma and Adenocarcinoma Gastrointestinal PDOs

ID	Catalog Number	Age	Sex	Organ	Disease Type or Normal
14-881-CR	SCC310	46	M	Colon (Rectum)	Adenocarcinoma (TP53 Pro151Ser, KRAS Gly12Asp, APC Pro1443fs) (RNA-seq)
ht-131-C	SCC328	58	F	Colon	Adenoma
CSC.240	SCC721	83	M	Colon	Adenocarcinoma
POP.024.G4	SCC728	66	M	Colon	Adenocarcinoma, metastatic (liver)
CSC.073.G1	SCC729	67	M	Colon	Adenocarcinoma, metastatic (lung)
POP.074.G4	SCC730	86	M	Colon	Adenocarcinoma
POP.112.G2	SCC731	74	M	Colon	Adenocarcinoma
POP.161.G1	SCC732	60	F	Colon	Adenocarcinoma, metastatic (lung)
POP.141.G2	SCC733	57	M	Colon	Adenocarcinoma, metastatic (liver)
POP.174.G1	SCC734	56	M	Colon	Adenocarcinoma, metastatic (lung)
POP.170	SCC735	61	M	Colon	Adenocarcinoma, metastatic (liver)
POP.066.G4	SCC736	46	F	Colon	Adenocarcinoma, metastatic (liver)
POP.092.G2	SCC737	46	F	Colon	Adenocarcinoma
POP.160.G2	SCC738	69	M	Colon	Adenocarcinoma, metastatic (liver)
CSC.171C.G1	SCC739	47	F	Colon	Adenocarcinoma
CSC.171L.G1	SCC740	47	F	Colon	Adenocarcinoma, metastatic (liver)
CSC.413	SCC741	43	M	Colon	Adenocarcinoma
CSC.456	SCC742	68	M	Colon	Adenocarcinoma, metastatic (liver)
CSC.463	SCC743	43	F	Colon	Adenocarcinoma, metastatic (lung)

Colorectal Cancer Gastrointestinal PDOs

ID	Catalog Number	Age	Sex	Organ	Disease Type or Normal
ISO34	SCC500*	N/A	N/A	Sigmoid Colon	Colorectal Cancer (APC ∆E1451*, BRAF ∆K601E)
ISO38	SCC501*	N/A	N/A	Sigmoid Colon	Colorectal Cancer (TP53 Pro151Ser, KRAS Gly12Asp, APC Pro1443fs)
ISO48	SCC502*	N/A	N/A	Caecum	Colorectal Cancer (PIK3CA ∆E542K, CTNNB1 ∆S33C)
ISO49	SCC503*	N/A	N/A	Lower Sigmoid Colon	Colorectal Cancer (APC ∆R1450*A1446Lfs*27, TP53 ∆R248Q, KRAS ∆G12D, PIK3CA ∆E542K, FBXW7 ∆R465C)
ISO50	SCC504*	N/A	N/A	Colon (Rectum)	Colorectal Cancer (APC ∆R232*E1286*, TP53 ∆R248Q", KRAS ∆G12D, SMAD4 ∆E526K, SMAD2 ∆S464*)
ISO57	SCC505*	N/A	N/A	Colon (Upper Rectum)	Colorectal Cancer (APC ∆Q978*, TP53 ∆R282W, KRAS ∆G13D)
ISO68	SCC506*	N/A	N/A	Sigmoid Colon	Colorectal Cancer (APC ∆Q1096*E1408*, TP53 ∆R248W, KRAS ∆G13D, SMAD4 ∆Q534*)
ISO72	SCC507*	N/A	N/A	Caecum	Colorectal Cancer (APC ∆Q1291*, KRAS ∆G12D, ERBB3 ∆A232V)

Crohn’s Disease and Ulcerative Colitis Gastrointestinal PDOs

ID	Catalog Number	Age	Sex	Organ	Disease Type or Normal
ht-206-CR	SCC365	24	F	Colon	Crohns (RNA-seq)
ht-206-I-CR	SCC366	24	F	Ileum	Crohns
ht-208-CR-CR	SCC367	23	M	Colon (Rectum)	Crohns (RNA-seq)
ht-506-I	SCC368	34	F	Ileum	Normal Margin to Crohns
ht-405-CA-UC	SCC369	36	F	Colon Ascending	Ulcerative Colitis

Lung Patient-Derived Organoids

Our authenticated portfolio of human patient-derived lung cancer organoids includes adenocarcinoma and squamous cell carcinoma organoid lines. The 3dGRO^® Human Lung Organoid biobank is derived from patient-derived xenograft, primary tumors, or metastatic patient tissues; the organoids express the major epithelial markers, adenocarcinoma markers, or squamous cell carcinoma markers.

Lung Cancer PDOs

Catalog Number	Model ID	Disease	Source	Mutations	Gender	Age	RNA-Seq
SCC600	LPTO.54	Lung Adenocarcinoma (Acinar)	Primary Tumor	-	Male	78	Link
SCC601	LPTO.85	Lung Adenocarcinoma (Mucinous)	Primary Tumor	KRAS G12D	Female	80	Link
SCC602	LPTO.126	Lung Adenocarcinoma (Solid)	Primary Tumor	-	Male	81	Link
SCC603	XDO.344	Lung Adenocarcinoma (Acinar/Solid)	PDX	KRAS G12C	Male	46	Link
SCC604	XDO.137	Lung Adenocarcinoma (Acinar/Papillary)	PDX	EGFR E746_A750del	Female	69	Link
SCC606	PDXO.4056	Lung Adenocarcinoma (Solid)	PDX	cMET AMP	Male	64	Link
SCC607	PDXO.149	Lung Squamous Cell Carcinoma	PDX	PIK3CA AMP	Male	62	Link
SCC608	PDXO.377	Lung Squamous Cell Carcinoma	PDX	PIK3CA AMP	Female	81	Link

Pancreatic Patient-Derived Organoids

We offer a 3dGRO^® pancreatic organoid biobank for disease modeling research applications that consists of tissue-derived human pancreatic cancer organoids (PDAC organoids). Our biobank includes highly characterized organoid lines from PDAC diseased patients with primary tumors containing wild-type and KRAS driver mutations.

PDAC Organoids

Catalog Number	ID	Disease	Source	Age	Sex	KRAS Mutation
SCC700	PPTO.02	Pancreatic Ductal Adenocarcinoma	Primary Tumor	62	M	G12D
SCC702	PPTO.13	Pancreatic Ductal Adenocarcinoma	Primary Tumor	73	M	G12R
SCC703	PPTO.26	Pancreatic Ductal Adenocarcinoma	Primary Tumor	81	M	G12V
SCC704	PPTO.30	Pancreatic Ductal Adenocarcinoma	Primary Tumor	73	M	G12D
SCC705	PPTO.36	Pancreatic Ductal Adenocarcinoma	Primary Tumor	76	F	G12D
SCC706	PPTO.46	Pancreatic Ductal Adenocarcinoma	Primary Tumor	66	F	G12R
SCC707	PPTO.49	Pancreatic Ductal Adenocarcinoma	Primary Tumor	55	M	G12V
SCC708	PPTO.61	Pancreatic Ductal Adenocarcinoma	Primary Tumor	60	F	Wild-Type
SCC709	PPTO.64	Pancreatic Ductal Adenocarcinoma	Primary Tumor	58	M	G12D
SCC710	PPTO.65	Pancreatic Ductal Adenocarcinoma	Primary Tumor	69	F	G12D
SCC711	PPTO.76	Pancreatic Ductal Adenocarcinoma	Primary Tumor	57	F	G12D
SCC712	PPTO.80	Pancreatic Ductal Adenocarcinoma	Primary Tumor	78	F	G12R
SCC713	PPTO.90	Pancreatic Ductal Adenocarcinoma	Primary Tumor	57	F	G12V
SCC714	PPTO.93	Pancreatic Ductal Adenocarcinoma	Primary Tumor	66	M	Q61H
SCC716	OCIP.174	Pancreatic Ductal Adenocarcinoma	Xenograph	59	F	Q61H
SCC717	OCIP.180	Pancreatic Ductal Adenocarcinoma	Xenograph	64	M	Wild-Type
SCC718	OCIP.255	Pancreatic Ductal Adenocarcinoma	Xenograph	66	F	Unknown
SCC719	OCIP.285	Pancreatic Ductal Adenocarcinoma	Xenograph	65	F	Q61L
SCC720	OCIP.335	Pancreatic Ductal Adenocarcinoma	Xenograph	74	M	Wild-Type