Alternatively, we offer ready-to-use, collagen-coated 96-well strips (Table 1).
Products that are compatible for the preparation of collagen gels are in listed Table 2.
Due to cross-linkage and sequence variability, as well as structural changes that can occur during isolation and purification, Collagen Type I (C8919) is a heterogeneous mixture rather than a pure compound. This variability, along with slight differences in the raw material, lends itself to shifts in the opacity of the solution from lot to lot. Products listed as sterile is tested for sterility prior to release for sale to our customers. No further filtration of such products is necessary. A milky or particulate appearance is not indicative of microbial contamination and does not affect product use.
The solubility specification for some collagen products, like C7661, is clear to hazy colorless solution with a few insolubles at 1 mg/mL in water with 2 μL acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.
The solvents that are compatible to dilute collagens are provided in Table 1.
What is the optimum concentration of collagen for coating?
This can occur but does not harm the product. When warmed to room temperature, the product will liquify. Please make sure it is well mixed prior to dilution for coating culture ware.
The collagen products that can be used to make gels are listed in Table 2.
We make no recommendations about the storage of plates after coating. Because several factors, such as humidity, may impact on the longevity of coated plates, customers should make their own determination as to whether plates can be stored and under which conditions. The product should not be exposed to temperatures above 50 °C.
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