These products are available in the US, Canada, and select European countries.
The epithelium is at the interface between the body of an organism and the external environment, covering all exterior surfaces and interior lumina. The diverse functions of epithelial cells include protection, absorption, transcellular transport, secretion, and sensation.
PromoCell® Renal Epithelial Cells are isolated from normal adult human tissues of the kidneys including whole kidney and renal cortex. Shortly after isolation, all human epithelial cells are cryopreserved at passage 2 (P2) using a proprietary serum-free freezing medium, Cryo-SFM. After thawing, each cryovial contains at least 500,000 viable cells. Human renal epithelial cells are also available as proliferating cell cultures generated from cryopreserved cells that have been thawed and cultured for three days in our labs.
The Renal Epithelial Cell Growth Medium 2 is a low-serum medium containing epinephrine and select growth factors. Renal Epithelial Cell Growth Medium 2 is optimized for growing primary human cells, but can also be used for the culture of porcine and mouse renal epithelial cells. The Renal Epithelial Cell Medium 2 is available in two formats: Medium (ready-to-use) and Medium Kit. The Medium (ready-to-use) option consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack (a set of individual vials with pre-measured supplements) allowing the user full control over the media formulation. Adding the SupplementMix or the SupplementPack to the Basal Medium constitutes the complete Renal Epithelial Cell Growth Medium. The Basal Medium (with or without phenol red), SupplementMix, and SupplementPack are all available for individual purchase.
Renal Epithelial Cell Growth Medium 2 is recommended for
The Renal Epithelial Cell Growth Medium 2 is a low-serum medium that contains all the growth factors and supplements necessary for optimized growth of human renal epithelial cells, including epinephrine (see the table below for supplemented medium details). Renal Epithelial Cell Growth Medium 2 does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
Storage and Stability of Renal Epithelial Cell Growth Medium 2
The following rigorous quality control tests are performed for each lot of Renal Epithelial Cells:
All lots of PromoCell® Renal Epithelial Cell Media are subjected to comprehensive quality control tests using primary human renal epithelial cells. Each lot is checked for growth-promoting activity, adherence rate, and typical morphology of the tested renal epithelial cells. Approved in-house lots of media are used as a reference. In addition, every lot of media is tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Intended Use of Renal Epithelial Cell Products
These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions please see appropriate MSDS.
Note: Due to their low serum content or the absence of serum, PromoCell® media are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead, we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.
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