04-817
Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal
culture supernatant, clone MC463, Upstate®
別名:
H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
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すべての画像(6)
About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
rabbit
品質水準
抗体製品の状態
culture supernatant
抗体製品タイプ
primary antibodies
クローン
MC463, monoclonal
化学種の反応性
human
メーカー／製品名
Upstate®
テクニック
ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
multiplexing: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
phosphorylation (pSer10)
遺伝子情報
human ... HIST1H3F(8968)
詳細
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine
特異性
Broad species cross-reactivity is expected
Histone H3 phosphorylated at Serine 10
免疫原
Peptide containing the sequence RK[pS]TG. in which pS corresponds to phospho-serine at position 10 of human histone H3
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463 is a rabbit monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p, is specificity verified using DB & Mplex, published in peer reviewed journals & validated in ChIP, IF, WB, ICC, ChIP-seq.
Western Blot Analysis:
A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A).
Beadlyte Histone-Peptide Specificity Assay:
A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.
Immunofluorescence:
Representative image from a previous lot.
Positive chromosome immunostaining for mitotic HeLa cells and A431 cells.
Chromatin Immunoprecipitation:
A previous lot was shown to be suitable for ChIP by an independent laboratory.
A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A).
Beadlyte Histone-Peptide Specificity Assay:
A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.
Immunofluorescence:
Representative image from a previous lot.
Positive chromosome immunostaining for mitotic HeLa cells and A431 cells.
Chromatin Immunoprecipitation:
A previous lot was shown to be suitable for ChIP by an independent laboratory.
品質
routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells
ターゲットの説明
17kDa
関連事項
Replaces: 05-817
物理的形状
Cultured supernantant in 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
04-817:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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