05-1531

Anti-TATA-box-binding protein Antibody, clone SL30-3-563

clone SL30-3-563, from mouse

• 別名: TATA box binding protein, Transcription initiation factor TFIID TBP subunit, TATA-binding factor, TATA sequence-binding protein, TATA-box binding protein N-terminal domain, TATA-box factor
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: SL30-3-563, monoclonal
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• テクニック: ChIP: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_003185
• UniProtアクセッション番号: P20226
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TBP(6908)

詳細

詳細

The TATA-box-binding protein (TBP) is a critical, eukaryotic transcription factor. TBP a part of the RNA polymerase II eukaryotic transcription complex and is required for the transcriptional initiation of RNA polymerases. TBP is considered to be a limiting factor for certain RNA promoters. Expression is ubiquitous, however the highest expression has been observed in testis and ovary. Mutations in TBP result in spinocerebellar ataxia type 17(SCA17), a cerebellar disorder classified as autosomal dominant cerebellar ataxia and characterized by atrophy to the cerebellar and cerebral regions, extrapyramidal signs, and dementia.

特異性

This antibody recognizes TATA-box-binding protein at the N-terminus.

免疫原

Epitope: N-terminus

GST-tagged recombinant protein corresponding to human TATA-box-binding protein at the N-terminus.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
転写因子

Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in U2OS cells. (Denisso, S., et al. (2007). The EMBO Journal. 26:944–954.)

Use Anti-TATA-box-binding protein Antibody, clone SL30-3-563 (mouse monoclonal antibody) validated in WB to detect TATA-box-binding protein also known as TATA box binding protein, Transcription initiation factor TFIID TBP subunit.

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected TATA-box-binding protein on 10 µg of HeLa cell lysate.

ターゲットの説明

~38 kDa observed.

物理的形状

Protein G

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable