05-345

Anti-p21/WAF1/Cip1 Antibody

Upstate^®, from mouse

• 別名: CDK-interacting protein, CDK-interaction protein, DNA synthesis inhibitor, Melanoma differentiation-associated protein 6, cyclin-dependent kinase inhibitor 1A, melanoma differentiation associated protein 6, wild-type p53-activated fragment 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: CP36, monoclonal; CP74, monoclonal
• application: IP; WB
• 化学種の反応性: mouse, human
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• citations: 89

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: CP36, monoclonal; CP74, monoclonal
• 化学種の反応性: mouse, human
• メーカー／製品名: Upstate^®
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NM_000389
• UniProtアクセッション番号: P38936
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CDKN1A(1026)

詳細

詳細

Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically. The biochemical activities and patterns of expression of CKIs during development implicate these proteins as the primary effectors of signaling pathways that control cell cycle exit, an event that is critical for differentiation. Studies have shown that p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is a relatively poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb, which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.

特異性

Other species cross-reactivity is unknown.

Recognizes Cip1, MW 21 kDa

免疫原

Epitope: amino acids 1-80

Recombinant full-length human Cip1 with a histidine tag.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Immunoprecipitation: 4 μg of a previous lot immunoprecipitated Cip1 from 500 μg of HeLa nuclear extract.

Use Anti-p21/WAF1/Cip1 Antibody (Mouse Monoclonal Antibody) validated in WB, IP to detect p21/WAF1/Cip1 also known as CDK-interacting protein or cyclin-dependent kinase inhibitor 1A.

品質

Evaluated by western blotting on HeLa nuclear extract.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected Cip1 in 20 μg of HeLa nuclear extract

ターゲットの説明

21 kDa

物理的形状

Protein G Purified

Format: Purified

Purified, pooled monoclonal mouse IgGs in buffer containing 0.1 M Trisglycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.

保管および安定性

Stable for 1 year at from date of receipt.

アナリシスノート

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable