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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
AE-4, monoclonal
化学種の反応性
bovine, human, plant
メーカー／製品名
Upstate®
テクニック
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
アイソタイプ
IgG2a
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
bovine ... H1-4(617854)
human ... H1-4(3008)
詳細
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
特異性
Predicted to stain nuclei from other animal and plant species.
This antibody recognizes histone H1, Mr 32-33 kDa. Doublet detected due to hyperphosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2B > H4 > H2A, as determined with recombinant protein.
免疫原
Nuclei from myeloid leukemia biopsy cells. Clone AE-4.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Immunocytochemistry:
5-20 µg/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.
ELISA:
Recommended.
5-20 µg/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.
ELISA:
Recommended.
Use Anti-Histone H1 Antibody, clone AE-4 (mouse monoclonal antibody) validated in ELISA, ICC, WB to detect Histone H1 also known as H1 histone family member 4, Histone H1b, histone 1 H1e, histone cluster 1 H1e.
品質
Routinely evaluated by immunoblot on lysates from an acid extract of HeLa cells.
Western Blot:
1-4 µg/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells.
Western Blot:
1-4 µg/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells.
ターゲットの説明
32-33 kDa (Histone H1); Doublet (hyperphosphorylated Histone H1); 17 kDa (other histone isoforms).
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2a in buffer containing PBS, pH 7.2, with 0.1% sodium azide. Frozen solution.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
Nuclear extracts from NIH3T3 or HeLa cells.
Nuclear extracts from NIH3T3 or HeLa cells.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
05-457:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Jung Ha Choo et al.
BMC genomics, 8, 479-479 (2008-01-02)
MacroH2A1 is a histone variant that is closely associated with the repressed regions of chromosomes. A recent study revealed that this histone variant is highly enriched in the inactive alleles of Imprinting Control Regions (ICRs). The current study investigates the
Nicotine induces chromatin remodelling through decreases in the methyltransferases GLP, G9a, Setdb1 and levels of H3K9me2.
Chase, KA; Sharma, RP
The International Journal of Neuropsychopharmacology null
Kwang-Hee Lee et al.
The Plant cell, 23(12), 4298-4317 (2011-12-14)
The regulatory particle (RP) of the 26S proteasome contains a heterohexameric ring of AAA-ATPases (RPT1-6) that unfolds and inserts substrates into the core protease (CP) for degradation. Through genetic analysis of the Arabidopsis thaliana gene pair encoding RPT2, we show
Super-resolution microscopy reveals decondensed chromatin structure at transcription sites.
Wang, Y; Maharana, S; Wang, MD; Shivashankar, GV
Scientific Reports null
Jae Hyun Lee et al.
Human molecular genetics, 18(14), 2567-2574 (2009-04-22)
We recently described two opposing states of transcriptional competency. One is termed 'competent' whereby a gene is capable of responding to trans-acting transcription factors of the cell, such that it is active if appropriate transcriptional activators are present, though it
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