07-225

Anti-TrkB Antibody

Upstate^®, from rabbit

• 別名: BDNF/NT-3 growth factors receptor, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, neurotrophic tyrosine kinase receptor, type 2, tyrosine kinase receptor B
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, rat
• メーカー／製品名: Upstate^®
• テクニック: western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_036863
• UniProtアクセッション番号: Q16620
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NTRK2(4915)

詳細

詳細

The Trk (tropomyosin-receptor-kinase) proto-oncogene family of receptor tyrosine kinases contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkB is the high-affinity to the binding brain-derived neurotrophic factor (BDNF) receptor and neurotrophin-4/5 NT-4/5). TrkB is autophosphorylated on five cytoplasmic tyrosines. The binding of BDNF to TrkB receptor causes many intercellular cascades to be activated such as promoting survival of sensory neurons mainly through Shc site-independent pathways.The signaling activity of these receptors includes the Ras – MAP kinase pathway, and the PI3 Kinase pathway. These receptors are responsible for resistance to apoptosis, obesity, Alzheimer’s disease, and mood disorders.

特異性

Recognizes TrkB, Mr 145 kDa, as well as an additional protein, Mr 90 kDa, which is likely a TrkB degradation product.

免疫原

The entire extracellular domain (corresponding to residues 1–429) of the rat TrkB receptor, expressed in COS cells. The immunizing sequence has 97% identity with mouse TrkB and 88% identity with human TrkB.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経化学・ニューロトロフィン

This Anti-TrkB Antibody is validated for use in WB for the detection of TrkB.

品質

Evaluated by western blot on mouse brain membrane protein preparations.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.

ターゲットの説明

145 κDa

物理的形状

Protein A purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.

保管および安定性

Stable for 1 year at -20ºC from date of receipt.

アナリシスノート

Control
Brain tissue.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1