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認証
APHA
FDA-BAM
ISO 22178
ISO 6888
品質水準
無菌性
non-sterile
形状
granular
包装
pack of 500 g
メーカー／製品名
GranuCult® prime
テクニック
microbe id | specific enzyme detection: suitable
microbiological culture: suitable
pH
6.8 (37 °C, 58 g/L in H2O)
溶解性
58 g/L
かさ密度
570 kg/m3
アプリケーション
food and beverages
microbiology
保管温度
15-25°C
適合性
Staphylococcus spp.
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関連するカテゴリー
詳細
GranuCult® Baird-Parker agar (base) is used for the isolation and enumeration of Staphylococcus aureus. This medium contains lithium chloride and tellurite to inhibit the growth of accompanying microbial flora, whereas pyruvate and glycine selectively stimulate the growth of staphylococci. Agar is the solidifying agent. The egg-yolk content makes the medium opaque. Staphylococcus colonies show two characteristic features when grown in this medium: (a) characteristic zones and rings are formed as a result of lipolysis and proteolysis, and (b) reduction of tellurite to tellurium produces a black coloration. The egg-yolk reaction and tellurite reduction usually occur together with a positive coagulase reaction and thus can serve as an index for the latter. According to EN ISO 6888-1, the addition of sulfamethazine is advised to suppress the growth and swarming of Proteus sp., if these are suspected in the test sample.
アプリケーション
GranuCult® Baird-Parker agar (base) is used for the isolation, presumptive identification, and enumeration of Staphylococcus aureus from food and animal feed as well as from other materials.
関連事項
Replaces: 1.05406.5007
アナリシスノート
Appearance (clearness): clear to turbid
Appearance (color): yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 50 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Staphylococcus saprophyticus ATCC 15305 (WDCM 00159)): no limit
Growth (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): no limit
Growth (Enterococcus hirae ATCC 8043 (WDCM 00089)): no limit
Growth (Proteus mirabilis ATCC 29906 (WDCM 00023)): no limit
Colonies (Staphylococcus aureus ATCC 6538 (WDCM 00032)): Black or grey colonies with clear halo
Colonies (Staphylococcus aureus ATCC 25923 (WDCM 00034)): Black or grey colonies with clear halo
Colonies (Staphylococcus saprophyticus ATCC 15305 (WDCM 00159)): Black or grey colonies without clear halo
Colonies (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): Black or grey colonies without clear halo
Colonies (Enterococcus hirae ATCC 8043 (WDCM 00086)): Black or grey colonies without clear halo
Colonies (Proteus mirabilis ATCC 29906 (WDCM 00023)): Brown or black colonies without clear halo
Incubation:
24 ± 2 to 48 ± 2 hours at 37 ± 1 °C aerobic
E.coli 48 ± 2 hours at 37 ± 1 °C aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar
Appearance (color): yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 50 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Staphylococcus saprophyticus ATCC 15305 (WDCM 00159)): no limit
Growth (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): no limit
Growth (Enterococcus hirae ATCC 8043 (WDCM 00089)): no limit
Growth (Proteus mirabilis ATCC 29906 (WDCM 00023)): no limit
Colonies (Staphylococcus aureus ATCC 6538 (WDCM 00032)): Black or grey colonies with clear halo
Colonies (Staphylococcus aureus ATCC 25923 (WDCM 00034)): Black or grey colonies with clear halo
Colonies (Staphylococcus saprophyticus ATCC 15305 (WDCM 00159)): Black or grey colonies without clear halo
Colonies (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): Black or grey colonies without clear halo
Colonies (Enterococcus hirae ATCC 8043 (WDCM 00086)): Black or grey colonies without clear halo
Colonies (Proteus mirabilis ATCC 29906 (WDCM 00023)): Brown or black colonies without clear halo
Incubation:
24 ± 2 to 48 ± 2 hours at 37 ± 1 °C aerobic
E.coli 48 ± 2 hours at 37 ± 1 °C aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar
その他情報
The prepared medium is clear to turbid and yellowish-brown.
脚注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法的情報
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Skin Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 3
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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B Tenderis et al.
International journal of food microbiology, 321, 108560-108560 (2020-02-23)
Foodborne illnesses affect the health of consumers worldwide, and thus searching for potential antimicrobial agents against foodborne pathogens is given an increased focus. This research evaluated the influence of sodium lactate (SL), encapsulated (e) and unencapsulated (u) polyphosphates (PP; sodium
資料
Microbial culture media is available in both powdered and granulated forms. This article compares the characteristics of each culture media format with regards to safety, handling and convenience.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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