おすすめの製品
由来生物
rabbit
品質水準
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
mouse, human
メーカー／製品名
ChIPAb+
Upstate®
テクニック
ChIP: suitable
dot blot: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
遺伝子情報
human ... H3F3B(3021)
詳細
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Histone H3.3 set includes the Histone H3.3 antibody, a Normal rabbit IgG, and control primers which amplify a 87 bp region of ChIP Primers, GAPDH Coding Region D2 Human. The Histone H3.3 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H3.3 -associated chromatin.
The ChIPAb+ Histone H3.3 set includes the Histone H3.3 antibody, a Normal rabbit IgG, and control primers which amplify a 87 bp region of ChIP Primers, GAPDH Coding Region D2 Human. The Histone H3.3 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H3.3 -associated chromatin.
特異性
Broad species cross-reactivity expected based on 100% sequence homology.
This antibody recognizes human Histone H3.
免疫原
Epitope: a.a. 85-105
KLH-conjugated linear peptide corresponding to human Histone H3.3.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal rabbit IgG or 2 µg Anti-Histone H3.3 and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of Histone H3.3 associated DNA fragments was verified by qPCR using ChIP Primers, GAPDH Coding Region D2 as a positive locus, and beta-actin promoter primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa acid extract was probed with Anti-Histone H3.3 (1 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Histone H3.3 (~17 kDa). (Figure 3).
Dot Blot Analysis;
Representative lot data.
Recombinant H3.2 and H3.3 spotted on PVDF (1, 10, 100, 1,000 ng histone per slot). Detection with 0.5 µg/mL a-H3.3 antibody in TBS-T, 5% milk. (Figure 4).
Image courtesy of Simon Elsässer, Laboratory of David Allis, Rockefeller University, New York.
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal rabbit IgG or 2 µg Anti-Histone H3.3 and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of Histone H3.3 associated DNA fragments was verified by qPCR using ChIP Primers, GAPDH Coding Region D2 as a positive locus, and beta-actin promoter primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa acid extract was probed with Anti-Histone H3.3 (1 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Histone H3.3 (~17 kDa). (Figure 3).
Dot Blot Analysis;
Representative lot data.
Recombinant H3.2 and H3.3 spotted on PVDF (1, 10, 100, 1,000 ng histone per slot). Detection with 0.5 µg/mL a-H3.3 antibody in TBS-T, 5% milk. (Figure 4).
Image courtesy of Simon Elsässer, Laboratory of David Allis, Rockefeller University, New York.
This ChIPAb+ Histone H3.3 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
包装
25 assays per set. Recommended use: ~2 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
品質
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG or 2 µg of Anti-Histone H3.3 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of Histone H3.3 associated DNA fragments was verified by qPCR using ChIP Primers, GAPDH Coding Region D2 (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG or 2 µg of Anti-Histone H3.3 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of Histone H3.3 associated DNA fragments was verified by qPCR using ChIP Primers, GAPDH Coding Region D2 (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
ターゲットの説明
~17 kDa observed
物理的形状
Affinity purified
Anti-Histone H3.3 (rabbit polyclonal). One vial containing 50 µg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Store at -20° C.
Concentration: 0.7 mg/mL
Normal Rabbit IgG. One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
Control Primers, GAPDH Coding Region D2 Human. One vial containing 75 μL of 5 μM of each primer specific for human GAPDH coding region. Store at -20°C.
FOR: GCC ATG TAG ACC CCT TGA AGA G
REV: ACT GGT TGA GCA CAG GGT ACT TTA T
Concentration: 0.7 mg/mL
Normal Rabbit IgG. One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
Control Primers, GAPDH Coding Region D2 Human. One vial containing 75 μL of 5 μM of each primer specific for human GAPDH coding region. Store at -20°C.
FOR: GCC ATG TAG ACC CCT TGA AGA G
REV: ACT GGT TGA GCA CAG GGT ACT TTA T
保管および安定性
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
アナリシスノート
Control
Includes normal rabbit IgG and primers specific for human GAPDH coding region.
Includes normal rabbit IgG and primers specific for human GAPDH coding region.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
17-10245:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Liquan Zhou et al.
Development (Cambridge, England), 144(3), 519-528 (2016-12-21)
During development from oocyte to embryo, genetic programs in mouse germ cells are reshaped by chromatin remodeling to orchestrate the onset of development. Epigenetic modifications of specific amino acid residues of core histones and their isoforms can dramatically alter activation
Analysis of neonatal brain lacking ATRX or MeCP2 reveals changes in nucleosome density, CTCF binding and chromatin looping.
Kernohan, KD; Vernimmen, D; Gloor, GB; Berube, NG
Nucleic Acids Research null
William Chang et al.
Epigenetics & chromatin, 16(1), 4-4 (2023-01-27)
Cellular differentiation is marked by temporally and spatially coordinated gene expression regulated at multiple levels. DNA methylation represents a universal mechanism to control chromatin organization and its accessibility. Cytosine methylation of CpG dinucleotides regulates binding of methylation-sensitive DNA-binding transcription factors
Joseph Aaron Goldman et al.
Developmental cell, 40(4), 392-404 (2017-03-02)
Chromatin regulation is a principal mechanism governing animal development, yet it is unclear to what extent structural changes in chromatin underlie tissue regeneration. Non-mammalian vertebrates such as zebrafish activate cardiomyocyte (CM) division after tissue damage to regenerate lost heart muscle.
Renee J Tamming et al.
Cell reports, 31(13), 107838-107838 (2020-07-02)
ATRX gene mutations have been identified in syndromic and non-syndromic intellectual disabilities in humans. ATRX is known to maintain genomic stability in neuroprogenitor cells, but its function in differentiated neurons and memory processes remains largely unresolved. Here, we show that
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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