196418

BAPTA, Tetrasodium Salt

Ca2+ chelator exhibiting a 105-fold greater affinity for Ca2+ .

• 別名: BAPTA, Tetrasodium Salt, 1,2- bis( o-Aminophenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid, 4Na, 1,2-bis(o-Aminophenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid, 4Na
• 実験式（ヒル表記法）: C₂₂H₂₀N₂O₁₀ · 4Na
• CAS番号: 126824-24-6
• 分子量: 564.36
• MDL番号: MFCD00067691
• UNSPSCコード: 12352106
• NACRES: NA.25

特性

• 品質水準: 100
• 形状: solid
• 反応適合性: reagent type: chelator
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 色: off-white
• 溶解性: water: 10 mg/mL
• 輸送温度: ambient
• 保管温度: −20°C
• InChI: 1S/C22H24N2O10.4Na/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32;;;;/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32);;;;/q;4*+1/p-4
• InChI Key: ZWSMLJACYSGFKD-UHFFFAOYSA-J

詳細

詳細

Ca²⁺-chelator exhibiting a 10⁵-fold greater affinity for Ca²⁺ (110 nM) than for Mg²⁺. After BAPTA complexes with Ca²⁺, the UV spectrum shifts from 254 to 279 nm at pH >6.0. Useful for spectrophotometric monitoring of extracellular Ca²⁺ levels, especially transient phenomena, because of its insensitivity to pH. Has been used for the measurement and control of Ca²⁺ levels in eggs, chromaffin cells, muscle cells, retinal cells, and pancreatic cells. In conjunction with certain formazan dyes, BAPTA can be used in a colorimetric assay for Mg²⁺. Has neuroprotective effects in vitro against excitotoxic damage and spontaneous recurrent seizures, such as epilepsy.

警告

Toxicity: Irritant (B)

再構成

Following reconstitution, aliquot, purge with inert gas and freeze (-20°C). To increase stability in solution, store at a high pH (>10). Stock solutions are stable for up to 1 month at -20°C.

その他情報

DeLorenzo, R.J., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14482.
Li, L., et al. 1995. J. Cell Physiol. 163, 105.
Tymianski, M., et al. 1994. J. Neurophysiol. 72, 1973.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable