268298

DAPI, Dihydrochloride

Cell-permeable DNA-binding dye.

• 別名: DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl
• 実験式（ヒル表記法）: C₁₆H₁₅N₅ · 2HCl
• CAS番号: 28718-90-3
• 分子量: 350.25
• MDL番号: MFCD00012681
• UNSPSCコード: 12352200
• NACRES: NA.54

特性

• 品質水準: 100
• アッセイ: ≥90% (HPLC)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 色: yellow
• 溶解性: water: 5 mg/mL; ethanol: soluble; methanol: soluble
• 蛍光検出: λ_ex ~359 nm; λ_em ~461 nm
• 輸送温度: ambient
• 保管温度: −20°C
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI Key: FPNZBYLXNYPRLR-UHFFFAOYSA-N

詳細

詳細

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H₂O (Cat. No. 508741).

Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Binds preferentially to DNA rich in adenine and thymine

Product does not compete with ATP.

Reversible: yes

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

その他情報

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H315,H317,H335
• 注意書き: P261 - P264 - P271 - P272 - P280 - P302 + P352
• 危険有害性の分類: Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable