324682

Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

• 別名: Elastase, Porcine Pancreas, High Purity, Crystallized
• CAS番号: 39445-21-1
• Enzyme Commission number: 3.4.21.36 (BRENDA, IUBMB)
• MDL番号: MFCD00130998
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 品質水準: 100
• フォーム: lyophilized
• 比活性: ≥25 units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated
• 溶解性: distilled water: 10 mg/mL
• その他の活性: Chymotrypsin ≤0.2%
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α₂-macroglobulin.

Native elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will hydrolyze 1.0 mol of Suc-Ala-Ala-Pro-Abu-pNA (Cat no.324699) per min at 25°C, pH 8.0.

物理的形状

Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.

再構成

Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.

その他情報

Largman, C. et al. 1976. Biochemestry15, 2491.
Mandl, I. 1962. Methods in Enzymol.5, 665.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H315,H319,H334,H335
• 注意書き: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3