71091

Nova Taq Hot Start DNA Polymerase

Heat-activatable modified form of recombinant Taq DNA polymerase

• 別名: Hot-start Taq DNA polymerase, NovaTaq HS DNA polymerase, hot start PCR
• UNSPSCコード: 41106314
• NACRES: NA.54

特性

• 由来生物: bacterial (Thermus aquaticus)
• 品質水準: 100
• リコンビナント: expressed in E. coli
• フォーム: liquid
• 使用法: sufficient for 1000 reactions; sufficient for 200 reactions
• 特徴: dNTPs included: no; hotstart
• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze
• テクニック: PCR: suitable
• 入力: purified DNA
• 適合性: suitable for PCR
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

NovaTaq Hot Start DNA Polymerase is a chemically modified Taq DNA Polymerase, which is inactive at room temperature. This polymerase is a derived recombinant form of Thermus aquaticus DNA polymerase. The enzyme exhibits a 5′→3′ DNA polymerase activity and lacks 3′→5′ exonuclease activity. The enzyme provides improved specificity when compared to standard Taq polymerase and can eliminate the presence of nonspecific amplification such as primer-dimers and mis-primed products. The enzyme must be activated by heat treatment (7–10 min at 95°C), after which thermal cycling can proceed. NovaTaq Hot Start DNA Polymerase generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen^® Perfectly Blunt^®, Acceptor, and LIC Vector Kits. NovaTaq Hot Start DNA Polymerase is a component of NovaTaq Hot Start Master Mix Kit.

Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration: 5.0 U/μl
Endonuclease:None detected
Exonuclease: None detected
Amplification efficiency: Functional PCR
Storage: –20°

アプリケーション

NovaTaq Hot Start DNA Polymerase has been used in polymerase chain reaction (PCR) amplification. It is suitable for use in Hot Start routine PCR.

特徴および利点

• Higher PCR specificity and yield
• Improved low-copy target amplification
• Ambient temperature setup compatible with automation
• Target amplification of up to 5 kbp
• Ideal for quantitative and high-throughput PCR applications

構成

•250 U or 5 × 250 UNovaTaq Hot Start DNA Polymerase (5 U/µl)

•1.5 ml or 5 × 1.5 ml10X NovaTaq Hot Start Buffer•1.5 ml or 5 × 1.5 ml25 mM MgCl₂

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

単位の定義

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-³²P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl.

法的情報

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(℃): Not applicable