AB5458

Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep)

serum, Chemicon^®

• 別名: neprilysin, Neprilysin, Enkephalinase, enkephalinase, atriopeptidase, Atriopeptidase, Neutral Endopeptidase, NEP, Endopeptidase 24.11, CD10
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: IHC; WB
• 化学種の反応性: rat, mouse, human
• テクニック: immunohistochemistry: suitable; western blot: suitable
• citations: 26

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat, mouse, human
• メーカー／製品名: Chemicon^®
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NM_000902.3
• UniProtアクセッション番号: P08473
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MME(4311)

詳細

詳細

Human neutral endopeptidase (Neprilysin) is single pass type II integral membrane endopeptidase that functions to activate and degrade biologically active enzymes. Endopeptidase,in general, function to cleave peptide bonds within enzyme sequences, in contrast to exopeptidases that target the end sequence. Neprilysin is part of the peptidase M13 family that acts on polypeptides of less than 30 amino acids with Thermolysin-like specificity for cleave sites. Thermolysin is thought to cleave before amino acids Ile, Met, Phe, Trpm or Val (unless Pro precedes) and after Ala, Asp, His, or Thr. Neprilysin preferentially cleaves polypeptides between hydrophobic residues, particularly with Phe or Tyr at position 1. Neprilysin research has found it important in the degradation of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Research has found it may function in the degradation of atrial natriuretic factor (ANF). Upregulation of ANF functions to regulate homeostatis of water, sodium, potassium and adipose in response to high blood pressure in muscle cells of the heart.

特異性

Reactivity with other species has not been confirmed.

Recognizes Neprilysin (Neutral Endopeptidase, NEP). On western blots of rat kidney extracts the antibody detects a band migrating at approximately 86 kDa corresponding to neprilysin.

免疫原

Rat Neprilysin.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経変性疾患

This Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep) is validated for use in IH, WB for the detection of Neprilysin (Neutral Endopeptidase).

Western Blotting:
A previous lot of this antibody was used on Western Blotting.

Immunohistochemistry:
A previous lot of this antibody was used on Immunohistochemistry.

Optimal working dilutions must be determined by the end user.

ターゲットの説明

~86 kDa

物理的形状

Unpurified

Rabbit polyclonal serum. Contains no preservative.

保管および安定性

Stable for 1 year at -20ºC from date of receipt.

アナリシスノート

Control
Kidney tissue.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable