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由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
human
化学種の反応性(ホモロジーによる予測)
bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
包装
antibody small pack of 25 μg
テクニック
immunocytochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... NR4A2(4929)
詳細
Nuclear receptor subfamily 4 group A member 2 (UniProt: P43354; also known as Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor) and is encoded by the NR4A2 (also known as NOT, NURR1, TINUR) gene (Gene ID: 4929) in human. Nurr-1 belongs to the nuclear hormone receptor family that acts as a transcriptional regulator and is considered to be important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is also crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. It is expressed in a number of T cell, B cell and fibroblast cell lines, but its expression is higher in brain tissue. Two isoforms of Nurr-1 have been reported that are produced by alternative splicing. The ligand-binding domain (LBD) of Nurr-1 contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. It also lacks a classical binding site for co-activators. However, it has hydrophobic region on the surface of LBD, which is critical for its activity. (Ref.: Volakakis N et al (2006). J. Mol. Endocrinol 37(2); 317-326; Wang G et al. (2003). Nature 423(6939):555-560).
特異性
This rabbit polyclonal antibody detects NR4A2 (Nurr1) in human cells. It targets an epitope within 16 amino acids from the N-terminal region.
免疫原
Epitope: N-terminus
KLH-conjugated liner peptide corresponding to 16 amino acids from the N-terminal region of human Nuclear receptor subfamily 4 group A member 2 (Nurr1).
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Anti-Nurr1 (NR4A2), Cat. No. ABE1455, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 2 and has been tested for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nurr1 (NR4A2) in HeLa cells.
品質
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Nurr1 (NR4A2) in THP-1 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Nurr1 (NR4A2) in THP-1 cell lysate.
ターゲットの説明
~67 kDa observed. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Molecular neurobiology, 56(11), 7872-7887 (2019-05-28)
Nuclear receptor related 1 protein (Nurr1) is an important transcription factor required for differentiation and maintenance of midbrain dopaminergic (DA) neurons. Since decrease in Nurr1 function either due to diminished expression or rare mutation is associated with Parkinson's disease (PD)
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