MAB1900

Anti-Integrin β1D Antibody, CT cytoplasmic domain, clone 2B1

clone 2B1, Chemicon^®, from mouse

• 別名: CD29
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 2B1, monoclonal
• application: ICC; IHC; WB
• 化学種の反応性: canine, human, mouse
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 20

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 2B1, monoclonal
• 化学種の反応性: canine, human, mouse
• メーカー／製品名: Chemicon^®
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_002211.2; NM_033666.1; NM_033667.1; NM_033668.1; NM_033669.1; NM_133376.1
• UniProtアクセッション番号: P05556
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ITGB1(3688)

詳細

詳細

Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. There are two major forms of integrin beta 1:beta 1A and beta 1D which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle, which instead express the beta1D variant.

特異性

MAB1900 is specific for the cytoplasmic domain of integrin beta1D subunit. Integrin beta1D is present in cardiac and skeletal muscle. In proliferating C2C12 cells, beta1D is not expressed; C2C12 after differentiation into myotubes, however, express high levels of beta1D integrin. beta1D integrin was also found expressed in adult mouse extracts from heat, hind limb muscle, and tongue, with a faint band in skin. No reactivity was observed in extracts from lung, kidney, liver, or spleen {van der Flier et al, 1997}.

免疫原

Epitope: C-terminus cytoplasmic domain

Synthetic peptide including an appending N-terminal cysteine: CQENPIYKSPINNFKNPNYGRKAGL coupled to KLH.

アプリケーション

Research Category
細胞骨格

Research Sub Category
インテグリン

This Anti-Integrin β1D Antibody, C-terminus cytoplasmic domain, clone 2B1 is validated for use in WB, IC, IH for the detection of Integrin β1D.

Western Blotting: 1:100-1:1000; recognizes a 116kDa band in reduced, 7% PAGE-SDS westerns in differentiated C2C12 cells and some adult tissues {See van der Flier, 1997}. 1% SDS lysis buffer is recommended over RIPA or traditional reducing sample buffer.

Immunocytochemistry:

Immunohistochemistry: 1:25-1:200. Frozen, unfixed tissue sections, post-fixed with 2% PFA after primary and secondary antibody additions {van der Flier, 1997}. Alternatively, 4-7μm cyrosections fixed with cold acetone for 2 minutes and stored at -80°C until used. Airdried sections are rehydrated in PBS prior to use; detection via ABC systems is encouraged.

Optimal working dilutions must be determined by the end user.

物理的形状

Format: Purified

Purified immunoglobulin. Liquid in PBS containing 0.09% sodium azide.

保管および安定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable