MAB4603

Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03

clone 3CSP03, Chemicon^®, from mouse

• 別名: CPP32
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 3CSP03, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2a
• NCBIアクセッション番号: NM_004346.3; NM_032991.2
• UniProtアクセッション番号: P42574
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CASP3(836)

詳細

特異性

Recognizes a protein of 32 kDa, identified as the Caspase-3 protein. Highly specific to Caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two subunits of 17 kDa and 12 kDa respectively. Caspase-3 has been implicated in the induction of apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers, including Fas-mediated apoptosis.

免疫原

Epitope: large subunit & proform

Recombinant full-length human caspase-3 protein

アプリケーション

Research Category
アポトーシス及び癌

代謝

Research Sub Category
カスパーゼ

酵素及び生化学

Detect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P).

Western blot: 1 μg/ml for 2 hours at RT.

Immunoprecipitation: 2 μg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.

Optimal working dilutions must be determined by end user.

関連事項

Replaces: 04-440; 04-1088

物理的形状

Format: Purified

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

保管および安定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable