MABN72

Anti-DLG4 Antibody

mouse monoclonal, K28/86

• 別名: discs, large homolog 4 (Drosophila), discs large homolog 4, disks large homolog 4, Tax interaction protein 15, post-synaptic density protein 95, Postsynaptic density protein 95, Synapse-associated protein 90
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: K28/86, monoclonal
• application: IHC; IP; WB
• 化学種の反応性: rat
• テクニック: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 6

特性

• 製品名: Anti-pan-MAGUK Antibody, clone K28/86, clone K28/86, from mouse
• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: K28/86, monoclonal
• 化学種の反応性: rat
• 化学種の反応性（ホモロジーによる予測）: human (immunogen homology), mouse (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1λ
• NCBIアクセッション番号: NP_001356
• UniProtアクセッション番号: P78352
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DLG4(1742)

詳細

詳細

The MAGUKs are membrane-associated guanylate kinase related (MAGUK) proteins that are defined by their inclusion of PDZ, SH3 and GUK domains. The GUK domain that they have is structurally very similar to that of the guanylate kinases, which converts ATP into ADP. However, it is known to be catalytically inactive as the P-Loop which normally binds ATP is absent. It is thought that the MAGUKs have adopted the GUK domain for their own purposes, primarily based on its ability to form protein-protein interactions with cytoskeleton proteins, microtubule/actin based machinery and molecules involved in signal transduction. In addition many of the MAGUK proteins also contain regions homologous of CaMKII, WW and L27 domains. Some of the members of the MAGUK family of proteins include DLG4 (Disks large homolog 4), DLG2, PSD95, MPP1 and other cytoskeleton-interacting proteins.

特異性

This antibody exhibits cross reactivity against recombinant Chapsyn-110, SAP97, and SAP102. (UC Davis/NIH NeuroMab Facility, http://neuromab.ucdavis.edu)

免疫原

Recombinant protein corresponding to a fusion domain of human PSD95/SAP90 proteins.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経シグナリング

Anti-pan-MAGUK Antibody, clone K28/86 is an antibody against pan-MAGUK for use in IH, WB & IP.

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected the MAGUK family of proteins in rat cerebellum and rat hypothalamus tissues.

Immunoprecipitation Analysis: A previous lot of this antibody was successfully used by an independent laboratory in IP. (Rasband, M., et al. (2002). The Journal of Cell Biology. 159(4):663–672.)

品質

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected members of the MAGUK protein family on 10 µg of rat brain tissue lysate.

ターゲットの説明

~ 85-110 kDa observed

物理的形状

Protein G

Format: Purified

Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Rat brain tissue lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable