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Merck
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MRCF0R030

Millipore

Microcon®-遠心式フィルター

NMWCO 30 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL

別名:

Centrifuge concentrator

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About This Item

UNSPSCコード:
41104916
eCl@ss:
36100101
NACRES:
NB.24

物質

Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)

品質水準

400

無菌性

non-sterile

特徴

holdup volume> 10 μL

メーカー／製品名

Microcon®

パラメーター

0.5 mL sample volume

テクニック

DNA purification: suitable
RNA purification: suitable
protein extraction: suitable
protein purification: suitable

L

4.50 cm (1.8 in.)

直径

12.3 mm

ろ過面積

0.32 cm2

ポアサイズ

30 kDa NMWCO

輸送温度

ambient

関連するカテゴリー

詳細

1.5 mLチューブを使用できるすべての遠心分離機で、あらゆる高分子溶液を簡単かつ効率的に濃縮・脱塩します。低結合Ultracelメンブレンを用いたMicroconフィルターでは以下が可能です：
•希釈溶液であっても回収率は通常95%超
•最少量の検体であっても逆遠心して回収率を最大化
•ろ過したまたは濃縮したサンプルは標準的なマイクロ遠心チューブに保存できて便利
•濃縮係数は最大100X

特長：
•逆遠心により少量でも高い回収率（濃縮率20X未満)
•低結合Ultracelメンブレン
•速やかな処理

サンプルアプリケーション：
•法医学検査用のゲノムDNAの回収
•核酸の濃縮・脱塩（エタノール沈殿の代わりに使える、回収率の高い方法）
•増幅したDNAからのプライマーの除去
•フラットなメンブレンデザインを利用した簡単なろ液回収

関連事項

代替品：42410

法的情報

MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類コード

10-13 - German Storage Class 10 to 13

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

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Sarah K Stumper et al.
eLife, 8 (2019-02-06)
RNA polymerases (RNAPs) contain a conserved 'secondary channel' which binds regulatory factors that modulate transcription initiation. In Escherichia coli, the secondary channel factors (SCFs) GreB and DksA both repress ribosomal RNA (rRNA) transcription, but SCF loading and repression mechanisms are
Flavio Oliveira et al.
Toxins, 12(3) (2020-04-05)
Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species
Michael D Ward et al.
Clinical proteomics, 17, 11-11 (2020-03-21)
Detection of viral ribo-nucleic acid (RNA) via real-time polymerase chain reaction (RT-PCR) is the gold standard for the detection of Ebola virus (EBOV) during acute infection. However, the earliest window for viral RNA detection in blood samples is 48-72 h post-onset
Sergey Radko et al.
Biomedicines, 8(5) (2020-05-28)
Selected reaction monitoring (SRM) is a mass spectrometric technique characterized by the exceptionally high selectivity and sensitivity of protein detection. However, even with this technique, the quantitative detection of low- and ultralow-abundance proteins in blood plasma, which is of great
Chung-Hsuan Kao et al.
Molecular and cellular biology, 40(12) (2020-03-25)
Maintenance of protein homeostasis in eukaryotes under normal growth and stress conditions requires the functions of Hsp70 chaperones and associated cochaperones. Here, we investigate an evolutionarily conserved serine phosphorylation that occurs at the site of communication between the nucleotide-binding and
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資料

An Optimized Protocol for Peptide Mapping of Therapeutic Monoclonal Antibodies with Minimum Deamidation and Oxidation Artifacts

An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.

Simple Digestion Protocol for Proteomics Sample Prep

Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

Released N-Glycan Analysis of a Therapeutic Antibody Using BIOshell™ Glycan Column

This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.

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プロトコル

Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

Released N-Linked Glycan Analysis Workflow of Adalimumab

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

関連コンテンツ

Microcon® Centrifugal Filters Applications Guidelines & Specifications

Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.

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