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リコンビナント
expressed in E. coli
品質水準
フォーム
buffered aqueous glycerol solution
hydroxamate transferase activity
≥200 units/mg protein (biuret)
濃度
14 mg/mL
UniProtアクセッション番号
保管温度
−20°C
遺伝子情報
Pseudomonas aeruginosa PAO1 ... PA4163(880181)
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アプリケーション
Amidase from Pseudomonas aeruginosa has been used for testing its capability to hydrolyze ochratoxin A.
The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater .
生物化学的／生理学的作用
The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment .
単位の定義
1ユニットはpH 7.2、37°C、1分間で1.0 μmolのアセトアミドとヒドロキシルアミンを、アセトヒドロキシム酸およびアンモニアに変換する酵素量です。
物理的形状
50%グリセロール溶液(7 mM 2-メルカプトエタノール、リン酸バッファー塩含有)。
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
劇物
Jan Code
A6691-250UN:
A6691-BULK:
A6691-VAR:
A6691-1KU:
A6691-2KU:
Jorge Andrade et al.
The Journal of biological chemistry, 282(27), 19598-19605 (2007-04-20)
Microbial amidases belong to the thiol nitrilases family and have potential biotechnological applications in chemical and pharmaceutical industries as well as in bioremediation. The amidase from Pseudomonas aeruginosa isa6 x 38-kDa enzyme that catalyzes the hydrolysis of a small range
Butyramide-utilizing mutants of Pseudomonas aeruginosa 8602 which produce an amidase with altered substrate specificity.
J E Brown et al.
Journal of general microbiology, 57(2), 273-285 (1969-08-01)
Mo-Fei Li et al.
Fish & shellfish immunology, 32(2), 322-330 (2011-12-08)
Peptidoglycan recognition proteins (PGRPs) are a family of innate immune molecules that recognize bacterial peptidoglycan. PGRPs are highly conserved in invertebrates and vertebrates including fish. However, the biological function of teleost PGRP remains largely uninvestigated. In this study, we identified
Sebastian Zoll et al.
Journal of bacteriology, 194(15), 3789-3802 (2012-05-23)
The bifunctional major autolysin Atl plays a key role in staphylococcal cell separation. Processing of Atl yields catalytically active amidase (AM) and glucosaminidase (GL) domains that are each fused to repeating units. The two repeats of AM (R1 and R2)
Vikash Babu et al.
Colloids and surfaces. B, Biointerfaces, 89, 277-282 (2011-09-29)
In this study, enzymatic surface modification of polyacrylonitrile was studied using nitrile metabolizing enzyme of Amycolatopsis sp. IITR 215. During enzymatic treatment of polyacrylonitrile at pH of 5.8 and 7, it was observed that the conversion of cyano group to
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