C3868

抗カルセクエストリン2抗体 ウサギ宿主抗体

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗CASQ2抗体, 抗速筋・心筋型カルセクエストリン抗体
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~55 kDa
• 化学種の反応性: rat, human
• 化学種の反応性（ホモロジーによる予測）: mouse
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.0 mg/mL
• テクニック: western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2; western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction)
• UniProtアクセッション番号: O14958
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CASQ2(845) ; mouse ... Casq2(12373) ; rat ... Casq2(29209)

詳細

詳細

Calsequestrin is a major Ca²⁺ binding protein in cardiac and skeletal muscle.

Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.

免疫原

マウスカルセクエストリン1のアミノ酸57~74に相当する合成ペプチドのKLH結合体。この配列はヒトおよびラットのカルセクエストリン2で高度に保存されています(1アミノ酸が置換)。この配列はカルセクエストリン1には存在しません。

アプリケーション

Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.

Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)

生物化学的／生理学的作用

Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which functions as an internal Ca²⁺ store in the lumen of the sarcoplasmic reticulum (SR).

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)