C7464

モノクロナール抗サイクリンD1 マウス宿主抗体

clone DCS-6, ascites fluid

• MDL番号: MFCD00239664
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ARR; ICC; IHC (f); IHC (p); IP; WB
• クローン: DCS-6, monoclonal
• 化学種の反応性: rat, mouse, human, monkey
• citations: 25
• テクニック: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:200 using a human tumor cell line extract

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: DCS-6, monoclonal
• 分子量: antigen 36 kDa (several lower and higher molecular weight bands)
• 含みます: 15 mM sodium azide
• 化学種の反応性: rat, mouse, human, monkey
• テクニック: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:200 using a human tumor cell line extract
• アイソタイプ: IgG2a
• UniProtアクセッション番号: P24385
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CCND1(595) ; mouse ... Ccnd1(12443) ; rat ... Ccnd1(58919)

詳細

詳細

Monoclonal Anti-Cyclin D₁ (mouse IgG2a isotype) is derived from the DCS-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin D₁ protein is a G₁-phase cyclin in mammalian cells. This protein is a D-type cyclin. The CCD1 gene is located on the human chromosome at 11q13.3.

特異性

その他のD型サイクリンとの交差反応性はありません。

免疫原

リコンビナントヒトサイクリンD₁タンパク質。

アプリケーション

Monoclonal Anti-Cyclin D₁ antibody produced in mouse may be used in:

• immunoblotting
• immunofluorescence
• immunocytochemistry
• immunoprecipitation
• immunohistochemistry
• antibody-mediated knock-out experiments

生物化学的／生理学的作用

Cyclin D₁ is involved in regulating cyclin-dependent kinase (CDK) family members. Cyclin D₁ and D₂ are affiliated with CDK4 and/or CDK6 kinase activities that are observed in mid-G₁ and culminate in the late G₁ phase. Amplification of the cyclin D₁ gene is observed in a significant percentage of cancers, including breast, bladder carcinoma, mantle cell lymphomas, head and neck cancers. This gene is also amplified in esophageal squamous cell carcinomas.

物理的形状

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable