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製品種目
Duolink®
テクニック
proximity ligation assay: suitable
適合性
suitable for brightfield
suitable for fluorescence
保管温度
20-25°C
詳細
Duolink® In Situ Microplate Heat Transfer Block is an aluminium block ideal for keeping an even temperature across the plate during the incubation steps when using Duolink In Situ reagents in multiwell plates. Incubation in the pre-heated Heat Transfer Block increases staining efficiency and reproducibility between different plates, and reduces plate edge effects.
アプリケーション
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
Specificity
Duolink® In Situ Microplate Heat Transfer Block will increase staining efficiency and reproducibility between different plates and reduce edge effects when using Duolink® reagents in microtiter plates.
Keep the Heat Transfer Block preheated at 37°C and keep it in the 37°C incubator throughout the whole Duolink® In Situ protocol. Place the microtiter plate in the Heat Transfer Block during incubation steps.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Linkage
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Duolink® In Situ Microplate Heat Transfer Block will increase staining efficiency and reproducibility between different plates and reduce edge effects when using Duolink® reagents in microtiter plates.
Keep the Heat Transfer Block preheated at 37°C and keep it in the 37°C incubator throughout the whole Duolink® In Situ protocol. Place the microtiter plate in the Heat Transfer Block during incubation steps.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Linkage
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
特徴および利点
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
調製ノート
Storage and Stability: When not in use, store the Heat Transfer Block at room temperature.
法的情報
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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資料
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Duolink™アッセイプロトコルの準備・セットアップ・実施にあたり考慮すべき事項
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
実験のTips & Tricks、FAQ、基本的なトラブルシューティングなどのサポート情報。
関連コンテンツ
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
近接ライゲーションアッセイを用いたタンパク質間相互作用、翻訳後修飾、低発現タンパク質の検出、定量および可視化などの応用例
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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