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Merck

F5387

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

別名:

Goat Anti-Mouse IgG (Fc specific)–Fluorescein isothiocyanate

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46

由来生物

goat

品質水準

結合体

FITC conjugate

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

secondary antibodies

クローン

polyclonal

形状

buffered aqueous solution

保管条件

protect from light

テクニック

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
indirect immunofluorescence: 1:32

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

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詳細

IgG subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

特異性

Goat polyclonal anti-Mouse IgG (Fc specific)–FITC antibody detects all mouse IgG subclasses, it shows no reactivity with mouse IgG Fab fragment or human IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion against anti-goat IgG and anti-goat whole serum result in single arcs of precipitation. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC.

免疫原

Purified mouse IgG Fc fragment

アプリケーション

Anti-Mouse IgG (Fc specific)-FITC antibody has be used for
  • Immunofluorescence
  • Immunohistochemistry
  • Immunostaining
  • Confocal microscopy
  • Flow cytometry

生物化学的/生理学的作用

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections IgG is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Anti-Mouse IgG (Fc specific)-FITC antibody is specific for Fc fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG Fab fragment or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC.

その他情報

Antibody adsorbed with human IgG.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

調製ノート

Adsorbed to reduce background with human samples.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

nwg

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

F5387-.5ML:
F5387-1ML:
F5387-2ML:
F5387-VAR:
F5387-BULK:


試験成績書(COA)

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以前この製品を購入いただいたことがある場合

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文書ライブラリにアクセスする

Growth and stratification of epithelial cells in minimal culture conditions
Epidermal Cells, 25-43 (2010)
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Zika virus (ZIKV) has been defined as a teratogenic pathogen behind the increased number of cases of microcephaly in French Polynesia, Brazil, Puerto Rico, and other South American countries. Experimental studies using animal models have achieved tremendous insight into understanding
C Souriau et al.
Nucleic acids research, 25(8), 1585-1590 (1997-04-15)
Studies on receptor-ligand interactions are important for the design of agonists or antagonists of natural ligands. We developed a luciferase reporter assay to screen epidermal growth factor receptor (EGFR) binding molecules rapidly for their ability to stimulate or inhibit signal
The Soluble Heparin-Binding EGF-Like Growth Factor Stimulates EGF Receptor Trafficking to the Nucleus
Korotkevych NV, et al.
PLoS ONE, 10(5), e0127887-e0127887 (2015)
M Boutinaud et al.
Journal of dairy science, 95(1), 177-187 (2011-12-24)
To assess the regulation of mammary cell activity, survival, and proliferation by prolactin (PRL), 5 Holstein cows in early lactation received daily i.m. injections of 1mg of quinagolide, a suppressor of PRL release, for 9 wk, whereas 4 control cows

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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