F7367

抗ヤギIgG (全分子)–FITC ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Rabbit Anti-Goat IgG (whole molecule)–Fluorescein isothiocyanate
• MDL番号: MFCD00162348
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: FITC conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 保管条件: protect from light
• テクニック: direct immunofluorescence: 1:400; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

IgG antibodies are smallest and are found in all body fluids and capable of fighting bacterial and viral infection. It is the only type of antibody able to cross the placenta and immunise the foetus. The anti-goat IgG (whole molecule)-FITC antibody is useful in indirect immunofluorescence studies of immunised goat sera infected Trichostrongylus colubriformis (nematode). Antigens can be measured by quantitative immunofluorescence using image analysis, in tissue sections stained with FITC labelled rabbit anti goat IgG. Anti-goat IgG antibody reacts specifically with all goat immunoglobulins.

IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.

Immunoglobulin G (IgG) is found in blood and has four polypeptide chains. It contains two identical 25 kDa κ or λ light (L) chains and two identical 50 kDa γ heavy (H) chains. The light and heavy chains are interconnected by disulfide bonds. It is the most important type of glycoprotein antibody present in human serum, secreted by the B cells. IgG exists in four isotypes: IgG1, IgG2, IgG3, and IgG4. IgG has the longest serum half-life compared to other immunoglobulins.

免疫原

IgG purified from Goat

アプリケーション

Anti-Goat IgG (whole molecule)−FITC antibody has been used:

• in double labeling for laminin and α-fetoprotein (AFP) at a dilution of 1:400
• in immunohistochemistry at a dilution of 1:50
• in immunocytochemistry
• in immunochemical jagged-1 analyses
• in immunofluorescence at a dilution of 1:200

Anti-goat IgG antibody can be used in antisera localization for neurofilament antigens obtained in rat peripheral nerve and can be done in tissues of rat and human peripheral and central nervous system by the aid of indirect immunofluorescence.

生物化学的／生理学的作用

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Immunoglobulin G (IgG) stimulates the classical pathway of the complement system. Maternal IgG is transferred to the fetus through the placenta. This is vital for the immune defense of the neonate against infections. IgG regulates antibody-dependent cell-mediated cytotoxicity (ADCC) and might be associated with allergy. It nullifies virus particles and toxins. Digestion of IgG by papain produces fragment antigen-binding (Fab). Pepsin digestion of IgG generates crystallizable (fc) fragment and a single dimeric F(ab)2. IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be used as a possible tool to assess the severity and prognosis of coronavirus disease 2019 (COVID-19).

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)